miScript miRNA Mimics
For studies on miRNA function and gene regulation using synthetic miRNA
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Find or custom design the right target-specific assays and panels to research your biological targets of interest.
miScript miRNA Mimic
Cat. No. / ID: 219600
1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade) miScript miRNA Mimic, provided in tubes
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miScript miRNA Mimics are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.
Features
- Ready-to-transfect mimics behave like endogenous miRNAs
- Available for all human, mouse, and rat miRNAs in miRBase
- Highly flexible formats, scales, and grades
- Easy to order via GeneGlobe
Product Details
miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA Mimics are available at cell-culture grade (>90% purity) or animal grade (HPLC purified; for in vivo applications). For flexible screening, miScript miRNA Mimic Plates enable researchers to order any predesigned miScript miRNA Mimics in their choice of layout in 96-well or 384-well plates. miScript miRNA Inhibitors are suitable for similar experiments. For transfection, QIAGEN provides HiPerFect Transfection Reagent for miRNA Mimic transfection and Attractene Transfection Reagent for miRNA Mimic-DNA cotransfection.
For even higher performance in your miRNA studies, try our miRCURY LNA miRNA Mimics. These highly potent, mature miRNA mimics feature a unique, triple RNA strand design for superior results.
Performance
miScript miRNA Mimics cause a decrease in gene expression similar to that observed with endogenous miRNAs (see figure " Comparable downregulation by endogenous miRNA and miScript miRNA Mimic"). The observed decrease in gene expression is often mediated at the translational level (see figure " Downregulation at protein level after miR-1 Mimic transfection"). miScript miRNA Mimics efficiently inhibit gene expression for up to 72 hours after transfection (see figure " miScript miRNA Mimic stable for 72 hours").
See figures
Principle
Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs. Reduced gene expression after transfection of mimic provides evidence that the miRNA under study is involved in regulation of that gene. miScript miRNA Inhibitors can also be used for similar studies. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.
Procedure
miScript miRNA Mimics are available for every human, mouse, rat, and virus miRNA in the current version of miRBase, the microRNA database. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible.
Flexible formats enable either low-throughput analysis of small numbers of mimics or high-throughput screening of hundreds of mimics. miScript miRNA Mimics are available in tubes (1 nmol or 5 nmol) at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Mimics can be ordered at the 20 nmol scale (provided in tubes). For medium- to high-throughput applications, 96-well plates (1 nmol or 5 nmol), or 384-well plates (1 nmol) are available to suit your experimental requirements.
Applications
- miRNA research
- Gene regulation research
Supporting data and figures
Comparable downregulation by endogenous miRNA and miScript miRNA Mimic.
miR-1 is not endogenously expressed in HeLa S3 cells. miR-16 is endogenously expressed at high levels in HeLa S3 cells (Lagos-Quintana, M. et al. [2001] Identification of novel genes coding for small expressed RNAs. Science 294, 853.). Using HiPerFect Transfection Reagent, HeLa S3 cells (6 x 104 cells/well) were transfected with a luciferase reporter construct with a binding site for miR-1 in the 3' UTR; cotransfected with a luciferase reporter construct with a binding site for miR-1 in the 3' UTR and with a miR-1 Mimic; or transfected with a luciferase reporter construct with a binding site for miR-16 in the 3' UTR. Twenty-four hours later, luciferase activity was measured. miR-1 Mimic resulted in a similar decrease in luciferase level as endogenous miR-16.
Specifications
| Features | Specifications |
|---|---|
| Design | Predesigned and custom design avaliable |
| Species | Human, mouse, rat |
| Format | Single tubes, 96-well & 384 well plates |
| Scale | 1 nmol, 5 nmol, 20 nmol |
| Modification | None |
| Sequence provided | No |
Resources
Safety Data Sheets (1)
Kit Handbooks (4)
Brochures & Guides (3)
miRNA Research (1)
Certificates of Analysis (1)
FAQ
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
What kind of molecules are miScript miRNA Mimics?
Where can I find QIAGEN products for a specific gene or gene product?
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
What is the "seed sequence" of a miRNA?