Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Features
- High reliability using the internal control and positive controls
- Sensitive detection of as few as 57.3 copies/ml (QS-RGQ kit)
- Same temperature profile for multiple viruses (RG and QS-RGQ kits only)
Product Details
artus HSV-1/2 PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for rapid and sensitive detection of HSV-1/2 specific DNA isolated from CSF (RG kit), or from serum, plasma, CSF, and swabs (LC and TM kits). The artus HSV-1/2 LC, RG, and TM PCR Kits use automated or manual sample preparation and manual asssay setup techniques. The artus HSV-1/2 QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Performance
To ensure sensitivity, artus HSV-1/2 Kits have been optimized to detect low copy numbers of HSV-1/2 DNA. The analytical sensitivity of the artus HSV-1/2 QS-RGQ Kit is 57.3 copies/ml for HSV-1 and 65.7 copies/ml for HSV-2 in consideration of the purification and assay setup using the QIAsymphony RGQ (see figure " Sensitive detection of HSV-1 DNA").
| Kit | artus HSV-1/2 RG PCR Kit | artus HSV-1/2 QS-RGQ Kit |
|---|---|---|
| Sample type | Cerebral spinal fluid (CSF) | Cerebral spinal fluid (CSF) |
| Analytical sensitivity | 0.12 copies/μl HSV-1, 0.16 copies/μl HSV-2 in the PCR | 57.3 copies/ml HSV-1, 65.7 copies/ml HSV-2 in consideration of the purification |
| Specificity | Tested with HSV-1 (HF, KOS, MacIntyre strains) and HSV-2 (HG-52, G, and MS strains) | Tested with HSV-1 (HF, KOS, MacIntyre strains) and HSV-2 (HG-52, G, and MS strains) |
See figures
Principle
artus HSV-1/2 PCR Kits are based on the amplification and simultaneous detection of a specific region of the HSV-1 and HSV-2 genome using real-time PCR. The kits provide high levels of specificity and sensitivity (see figure " Sensitive detection of HSV-1 DNA").
The kits provide positive controls for HSV-1 and HSV-2 (see figures " Detection of the HSV-1 positive control" and "Detection of the HSV-2 positive control"). In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.
| Kit | artus HSV-1/2 RG PCR Kit and artus HSV-1/2 QS-RGQ Kit |
|---|---|
| Sample type | Cerebral spinal fluid (CSF) |
| Amplicon | 154 bp region of the UL5 gene |
See figures
Procedure
artus HSV-1/2 PCR Kits provide all necessary reagents optimized for reliable HSV-1/2 DNA detection and differentiation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
Common temperature profiles for multiple assays in one run
The temperature profiles of artus HSV-1/2 LC PCR, RG PCR, and QS-RGQ Kits correspond to the profiles of the artus LC PCR, RG PCR, and QS-RGQ Kits for CMV, EBV, VZV, and (RG and QS-RGQ kits only) BK virus. Therefore, these PCR assays can be performed and analyzed in a single run.
Complete automated system from sample to HSV-1/2 detection
The QIAsymphony RGQ workflow solution for HSV-1/2 detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HSV-1/2 QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure " Integrated QIAsymphony RGQ system for HSV-1/2 detection").
Recommendations for viral DNA purification when using artus LC, RG, and TM PCR Kits
For DNA purification, we recommend using the EZ1 DSP Virus Kit for automated viral nucleic acid purification.
See figures
Applications
artus HSV-1/2 PCR Kits enable rapid and sensitive detection of HSV-1/2 DNA from EDTA plasma. The kits enable discrimination of HSV-1 and HSV-2 using genotype specific probes.
The artus HSV-1/2 QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to HSV DNA detection and discrimination of HSV-1 and HSV-2.
Supporting data and figures
Detection of the HSV-1 positive control.
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Resources
Kit Handbooks (3)
Safety Data Sheets (1)
Certificates of Analysis (1)