artus HBV QS-RGQ Kit

• Complete IVD workflow
• High reliability using the internal control
• Highly sensitive detection of as few as 10.2 IU/ml
• Broad linear range from 31.6 to more than 20,000,000 IU/ml
• Accurate quantitation using the 5 standards supplied

To ensure highest sensitivity, the artus HBV QS-RGQ Kit has been optimized to detect low numbers of HBV DNA. The analytical sensitivity of the artus HBV QS-RGQ Kit is 10.2 IU/ml in consideration of the purification and assay setup using the QIAsymphony RGQ system (see figure " Highly sensitive detection of HBV DNA"). (1 IU/ml corresponds to 8.21 copies/ml for detection of HBV DNA on the Rotor-Gene Q. The conversion factor is an approximation based on an average factor across the assay's dynamic range.)

For highest specificity, validation of the artus HBV QS-RGQ Kit was carried out using various HBV isolates, including all genotypes A–H and related pathogens.

The artus HBV QS-RGQ Kit is based on the amplification and simultaneous detection of a specific region of the HBV genome using real-time PCR. The kit provides high levels of specificity, sensitivity (see figure " Highly sensitive detection of HBV DNA"), and reproducibility over a broad linear range.
Each artus HBV QS-RGQ Kit provides 5 HBV quantitation standards (see figure " Reliable quantitation of HBV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical HBV PCR is not reduced.

The artus HBV QS-RGQ Kit provides all necessary reagents optimized for reliable HBV DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

Complete automated system from sample to HBV detection

The QIAsymphony RGQ workflow solution for HBV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HBV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete IVD workflow (see figure " Integrated QIAsymphony RGQ system for HBV detection").

The artus HBV QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete IVD workflow from sample to HBV DNA detection and quantitation.