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AllPrep RNA/Protein Kit (50)
Cat. No. / ID: 80404
50 AllPrep Mini Spin Columns, 50 RNeasy Mini Spin Columns, 50 Protein Cleanup Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers
AllPrep RNA/Protein Kit ist für molekularbiologische Anwendungen vorgesehen. Dieses Produkt ist nicht für die Diagnose, Prävention oder Behandlung einer Krankheit bestimmt.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High-quality RNA and protein from the same sample
- Rapid purification with spin-column–based protocol
- Efficient sample preparation using less cell cultures
- High-performance RNA and native protein
Product Details
The AllPrep RNA/Protein Kit allows the simultaneous purification of total RNA and native protein from the same cultured cell sample.
Performance
The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. Analysis of purified RNA from a sample of cells on the Agilent 2100 Bioanalyzer typically gives RIN values of 10, indicating intact RNA. The native protein purified from the same cell sample contains all cellular proteins (see figure “ Highly intact RNA and high-quality protein from the same cell sample”). RNA purified with the AllPrep RNA/Protein Kit is suitable for real-time RT-PCR (see figure " Sensitivity in real-time RT-PCR") and purified cellular proteins demonstrate functionality in downstream enzyme assays (see figure " Purification of functional protein").
See figures
Principle
The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. For experiments to validate siRNA-mediated knockdown at the mRNA and protein levels, the kit streamlines sample preparation and allows the number of cell cultures to be significantly reduced. In addition, knockdown analysis is more reliable since RNA and protein come from the same sample.The AllPrep RNA/Protein Kit is part of QIAGEN’s range of closely integrated products for RNAi and gene expression analysis. These products provide highly reproducible and sensitive results. Products include optimized PCR master mixes, ready-to-run assays, siRNAs, and transfection reagents. Standardization of RNAi and gene expression analysis workflows by these products have contributed to success in many areas of biomedical research.
Procedure
The spin-column–based procedure enables purification of RNA and protein from a single cell sample in less than 40 minutes (see flowchart " AllPrep RNA/Protein procedure"). A method is available for simultaneous purification of DNA, RNA, and protein (suitable for western blotting, not for enzyme assays) from cultured cells and tissues using the AllPrep DNA/RNA/Protein Kit.
See figures
Applications
The AllPrep RNA/Protein Kit allows purification of RNA and protein from a single cell culture. The purified RNA and protein provide high performance in downstream applications, such as real-time RT-PCR, western blotting, and enzyme assays. (Note: The functionality of any protein of interest in an assay has to be tested according to its properties.)
Supporting data and figures
Highly intact RNA and high-quality protein from the same cell sample.
Total RNA was purified from HeLa cells and analyzed on the Agilent 2100 Bioanalyzer. The rRNA ratio (28S/18S) was 2.04. Total protein was purified from HeLa cells and analyzed by western blot. Analysis of duplicate protein samples with antibodies against Lamin (numclear protein) and TIM23 (mitochondrial protein) demonstrates that the total protein fraction contains proteins from subcellular compartments.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR, blotting, microarray |
| Elution volume | 30–50 µl |
| Format | Spin column |
| Processing | Manual (centrifugation) |
| Technology | Silica technology |
| Time per run or per prep | 40 minutes |
| Sample amount | 700 µl |
| Main sample type | Cultured cells |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA, native protein |
Resources
Kit Handbooks (2)
Safety Data Sheets (2)
Brochures & Guides (1)
Technical Information (1)
Quick-Start Protocols (1)
FAQ
What is the composition of Buffer APL?
Can the AllPrep RNA/Protein Kit be used with tissue?
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?