QIAprep Spin Miniprep Columns

• Ready-to-use plasmid DNA in minutes
• Reproducible yields of molecular biology grade plasmid DNA
• Single protocol for high- and low-copy vectors

The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

QIAprep Spin Miniprep standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. Learn more.

The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Column handling options  A,  B, and  C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

Purified DNA can be used in restriction digestion (see figure " Complete digestion with various restriction enzymes"). 

Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart " The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.

The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:

• PCR
• Restriction digestion
• Ligation and transformation
• Sequencing
• Screening

Compatible with Gilson's TRACKMAN Connected system

QIAGEN has programmed QIAprep Spin Miniprep protocols into the TRACKMAN Connected system, which controls the Bluetooth-enabled PIPETMAN M Connected pipettes sold by Gilson.

TRACKMAN Connected guides researchers through the QIAprep Spin Miniprep protocols while automatically adjusting pipette settings. Each step of the protocol execution is recorded to accelerate reporting, because the system generates a comprehensive run report.