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EZ1&2 DNA Blood 200 µl Kit (48)
Cat. No. / ID: 951034
For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)
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Sample volume
200 µl
350 µl
EZ1&2 DNA Blood Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Rapid purification of up to 12 µg DNA from whole blood
- Purification of up to 14 µg DNA from buffy coat using the EZ1&2 DNA Blood 350 µl Kit
- Processing of 200–350 µl whole blood samples
- Process up to 24 samples at the same time for easy intra-run comparability
Product Details
EZ1&2 DNA Blood Kits contain all required reagents and labware for rapid, automated purification of DNA from 200-350 μl whole blood samples or buffy coat samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.
EZ1&2 DNA Blood Kits are automated with the EZ2 Connect (1-24 samples per run). These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).
Performance
The automated procedure provides high repeatability (see figures "High-quality DNA from whole blood" and "Reproducible yields of high-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using EZ1&2 DNA Blood Kits gives high-performance results in downstream applications, such as PCR (see figure "High-quality DNA for sensitive and specific analyses").
See figures
Principle
Automated purification with EZ1&2 DNA Blood Kits requires use of either EZ2 Connect instruments or EZ1 instruments and EZ1 DNA Cards (see table “EZ1&2 DNA Blood Kits and EZ1 DNA Cards”).
EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.
EZ1&2 DNA Blood Kits and EZ1 DNA Cards
| EZ1&2 DNA Blood Kit | EZ1 DNA Card |
|---|---|
| EZ1&2 DNA Blood 200 µl Kit | EZ1 Advanced XL DNA Blood Card |
| EZ1&2 DNA Blood 350 µl Kit | EZ1 Advanced XL DNA Blood Card EZ1 Advanced XL DNA Buffy Coat Card |
Procedure
Whole blood samples (200 µl or 350 µl) are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.
Applications
The high-quality blood obtained using EZ1&2 DNA Kits is suited for use in many applications, such as:
- Population genetics and pharmacogenomic research
- PCR and real-time PCR
- Sequencing
- Oncology research
- Genetic identity testing and forensics
- Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies
Comparison of EZ1&2 DNA Blood Kits
| Features | EZ1&2 DNA Blood 200 µl Kit | EZ1&2 DNA Blood 350 µl Kit |
|---|---|---|
| Applications | PCR, real-time RT-PCR, genotyping | PCR, real-time RT-PCR, genotyping |
| Elution volume | 50, 100, 200 µl | 50, 100, 200 µl |
| For automated processing | EZ2 Connect, EZ1 Advanced XL | EZ2 Connect, EZ1 Advanced XL |
| Format | Sample tube | Sample tube |
| Main sample type | Whole blood | Whole blood |
| Processing | Automated | Automated |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | DNA | DNA |
| Sample amount | 200 µl | 350 µl |
| Technology | Magnetic particles | Magnetic particles |
| Time per run or per prep | 15–20 minutes | 15–20 minutes |
| Yield | 2.6–6 µg | 5–12 µg |
Supporting data and figures
High-quality DNA from whole blood.
DNA was purified from 200 µl blood samples using the EZ1&2 DNA Blood 200 µl Kit (upper lanes) and from 350 µl blood samples using the EZ1&2 DNA Blood 350 µl Kit (lower lanes). Lanes 1–6 and 7–12 are from the first and last of 8 processing runs on the EZ1 instrument, respectively. M: 1000 bp DNA ladder (100 ng); +: positive control; -: negative control. 2 µl (1%) of each eluate was visualized on the agarose gel.
Resources
Quick-Start Protocols (2)
Supplementary Protocols (1)
Kit Handbooks (2)
Brochures & Guides (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
Is training of lab personnel included with the purchase of a BioRobot EZ1?
How should I prepare buffy coat samples for use on the BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
Where can I find information about the worktable setup on the BioRobot EZ1?
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
How do I safely inactivate biohazardous flow-through material?
What disposables are required for nucleic acid isolations on the BioRobot EZ1?