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QIAamp 96 DNA Swab BioRobot Kit

Para la purificación automatizada de ADN de alto rendimiento a partir de hisopos

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QIAamp 96 DNA Swab BioRobot Kit (12)

Cat. No. / ID:   965842

Para 12 × 96 preparaciones de ADN: 12 placas QIAamp 96, tampones, QIAGEN Proteinase K, hojas de tiras AirPore, almohadilla de cinta, S-Blocks, gradillas con microtubos de recogida (1,2 ml), tapas
10.105,00 AUD
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El QIAamp 96 DNA Swab BioRobot Kit está concebido para su uso en aplicaciones de biología molecular. Este producto no está concebido para el diagnóstico, la prevención ni el tratamiento de enfermedades.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Purificación rápida de ADN de gran calidad
  • Sin extracción orgánica ni precipitación con alcohol
  • Rendimiento constante y de alto nivel
  • Eliminación de contaminantes e inhibidores

Product Details

El QIAamp 96 DNA Swab BioRobot Kit ofrece una purificación de ADN automatizada con hasta 192 hisopos en el BioRobot Universal System con el uso de la tecnología de membrana de sílice QIAamp de eficacia comprobada. Se procesan hasta 192 muestras en menos de 2,5 horas. El procedimiento está optimizado para su uso con hisopos secados al aire con varillas de plástico y algodón o puntas DACRON, aunque se pueden usar otros tipos de hisopo.

Performance

La tecnología QIAamp 96 de eficacia comprobada proporciona uniformidad entre pocillos en la recuperación de ADN, así como pureza sin contaminación cruzada entre muestras (consulte la figura « Prueba de contaminación cruzada»). La eliminación efectiva de inhibidores enzimáticos garantiza el rendimiento en aplicaciones posteriores, como los análisis TaqMan®, LightCycler y iCycler (consulte la figura « Rendimiento fiable»).

El ADN aislado con el QIAamp 96 DNA Swab BioRobot Kit está listo para su uso en una amplia variedad de aplicaciones de diagnóstico molecular y ciencia forense:

See figures

Principle

No se requiere extracción con fenol/cloroformo. El ADN se une de forma específica a la membrana de gel de sílice QIAamp mientras los contaminantes la atraviesan. Los inhibidores de la PCR, como los cationes divalentes y las proteínas, se eliminan por completo en cuatro eficientes pasos de lavado, dejando el ADN puro para su elución en agua o en un tampón incluido en el kit. La tecnología de QIAamp obtiene ADN de hisopos listos para su uso en procedimientos de PCR y blotting.

Procedure

El QIAamp 96 DNA Swab BioRobot Kit simplifica el aislamiento del ADN de hisopos con un procedimiento de placas de 96 pocillos rápido y automatizado (consulte el organigrama « Procedimiento con QIAamp 96 DNA Swab BioRobot»). El procedimiento está optimizado para su uso con hisopos secados al aire con varillas de plástico y algodón o puntas DACRON, aunque se pueden usar otros tipos de hisopo. El rendimiento habitual del QIAamp 96 DNA Swab BioRobot Kit es de 1–2 µg por hisopo (frotis bucales), con un volumen de elución de 150 µl.

La tecnología de preparación de muestras de QIAamp cuenta con licencia completa.

See figures

Applications

El QIAamp 96 DNA Swab BioRobot Kit ofrece la tecnología de QIAamp en un práctico formato de 96 pocillos para satisfacer las necesidades de purificación de alto rendimiento. Purifica el ADN de hasta 96 muestras de hisopos en menos de 2 horas. Tipos de muestras:

  • Frotis bucales
  • Frotis faríngeos
  • Hisopos oculares

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, secado
Yield1-2 µg por hisopo
FormatPlaca de 96 pocillos
For automated processingBioRobot Universal System, genotipado de BioRobot, BioRobot 9604
Sample amount1 hisopo por pocillo
ProcessingAutomatizado
Elution volume150 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinADN genómico
Main sample typeHisopos
TechnologyTecnología de sílice
Time per run or per prep<2 horas

Resources

Kit Handbooks (1)
For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604
Safety Data Sheets (1)
Certificates of Analysis (1)

Publications

Estrogen sulfation genes, hormone replacement therapy, and endometrial cancer risk.
Rebbeck TR; Troxel AB; Wang Y; Walker AH; Panossian S; Gallagher S; Shatalova EG; Blanchard R; Bunin G; DeMichele A; Rubin SC; Baumgarten M; Berlin M; Schinnar R; Berlin JA; Strom BL;
J Natl Cancer Inst; 2006; 98 (18):1311-20 2006 Sep 20 PMID:16985250
Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
Hanselle T; Otte M; Schnibbe T; Smythe E; Krieg-Schneider F;
Leg Med (Tokyo); 2003; 5 Suppl 1 :S145-9 2003 Mar PMID:12935575

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
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