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EZ1 DNA Investigator Cards

Para facilitar la configuración de protocolos para aplicaciones forenses en instrumentos EZ1

S_1084_5_GEN_V2

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EZ1 DNA Forensic Card

Cat. No. / ID:   9015864

Tarjeta preprogramada para los protocolos BioRobot EZ1 Forensic
1043,00 €
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Accessories
EZ1 DNA Card
EZ1 Advanced DNA Card
For
EZ1 DNA Tissue Kit
EZ1 DNA Investigator Kit
Las tarjetas EZ1 se utilizan en combinación con los EZ1 Kits en instrumentos EZ1. Consulte el manual específico del EZ1 Kit y el Manual de uso de BioRobot EZ1 para obtener información sobre el uso del producto.Las tarjetas EZ1 Advanced se utilizan en combinación con los EZ1 Kits en el EZ1 Advanced. Consulte el manual específico del EZ1 Kit y el Manual de uso de EZ1 Advanced para obtener información sobre el uso del producto.Las tarjetas EZ1 Advanced XL se utilizan en combinación con los EZ1 Kits en el EZ1 Advanced XL. Consulte el manual específico del EZ1 Kit y el Manual de uso de EZ1 Advanced XL para obtener información sobre el uso del producto.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Fácil configuración de protocolos con facilidad de uso de la tarjeta de crédito
  • El procesamiento estandarizado garantiza resultados reproducibles
  • Los protocolos optimizados ayudan a garantizar una purificación fiable
  • Seguridad del proceso avanzada mediante el registro de las muestras
  • Purificación automatizada de ADN genómico a partir de muestras de casos

Product Details

Las EZ1 DNA Investigator Cards están preprogramadas con protocolos para la purificación de ADN a partir de muestras forenses y de identidad humana utilizando el EZ1 DNA Investigator Kit en EZ1 Advanced, EZ1 Advanced XL o BioRobot EZ1.

Performance

Los EZ1 DNA Kits, cuando se utilizan en combinación con las EZ1 DNA Investigator Cards y los instrumentos EZ1, proporcionan una rápida purificación del ADN a partir de muestras forenses, por ejemplo, muestras de trazas, colillas de cigarrillos y pelo.

Manipulación más sencilla de materiales sólidos con el protocolo “tip dance”

Además, las EZ1 DNA Investigator Cards ofrecen un protocolo «tip dance» opcional y totalmente automatizado en los instrumentos EZ1, en el que la punta del filtro se mueve hacia delante y hacia atrás en relación con la plataforma de la mesa de trabajo mientras se pipetea. Esto permite procesar materiales sólidos, como hisopos, tejidos, plaquetas o colillas, directamente en el tubo de muestras. No es necesaria la centrifugación previa para eliminar los materiales sólidos que podrían obstruir la punta (consulte el diagrama de flujo  «Trazas y protocolo “tip dance” de EZ1 DNA Investigator»). El protocolo “tip dance” simplifica la manipulación y, en algunos casos, aumenta el rendimiento de ADN con una extracción más eficaz de la muestra (consulte la figura  «Procesamiento fácil y eficaz de materiales sólidos con el protocolo “tip dance”»).

Protocolo optimizado para volúmenes mayores de muestras

Un protocolo de gran volumen permite el procesamiento totalmente automatizado de volúmenes iniciales de hasta 500 µl en los instrumentos EZ1. Esto no solo permite una purificación eficaz del ADN a partir de muestras diluidas con bajas concentraciones de ADN, como las manchas difusas, sino que también permite la purificación a partir de muestras que requieren volúmenes mayores para una lisis completa. La capacidad de procesar mayores volúmenes de muestra, con el mismo volumen de elución que el protocolo de trazas estándar, permite mayores rendimientos de ADN más concentrado para una mayor sensibilidad en aplicaciones posteriores (véase  «Mayor rendimiento del ADN concentrado con el protocolo de gran volumen»).

See figures

Principle

Las EZ1 DNA Investigator Cards especifican una selección de protocolos de purificación sin necesidad de introducir datos manualmente. Se pueden adquirir tarjetas EZ1 adicionales en cualquier momento para aumentar la gama de aplicaciones de los instrumentos EZ1.

Procedure

Solo tiene que insertar la tarjeta EZ1 con el protocolo deseado en la ranura de la tarjeta e iniciar el instrumento EZ1.

Applications

El ADN de alta calidad que se obtiene mediante los instrumentos EZ1 con las EZ1 DNA Investigator Cards es apto para su uso en muchas aplicaciones, incluidas:

  • Pruebas de identidad genética y análisis forenses
  • Purificación de ADN a partir de muestras de trazas, como muestras de casos o de la escena de un crimen
  • Análisis rutinario de muestras de referencia
  • PCR multiplexada y real-time PCR
  • Análisis de genotipado, incluidas las tecnologías SNP, STR o NGS

Supporting data and figures

Resources

Quick-Start Protocols (2)
For use with the EZ1 instruments
For use with the EZ2 Connect Fx instrument
Supplementary Protocols (1)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Validation Reports (1)
For the EZ1 DNA Investigator Kit
  • BioRobot EZ1
  • EZ1 Advanced
  • EZ1 Advanced XL
Kit Handbooks (1)
For automated purification of DNA from forensic and human ID samples using EZ1 instruments
Brochures & Guides (1)
Fully automated low- to medium-throughput purification of nucleic acids
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
What EZ1 DNA Investigator protocol is recommended for very precious samples?
For precious samples, please use the Trace Protocol of the EZ1 DNA Investigator Card on the BioRobot EZ1.
FAQ ID -1154
When should the Normalization protocol be used?

The Normalization protocol is useful for routine, standardized analyses of reference samples, such as blood or buccal swabs, where sample-to-sample variability can result in widely varying DNA yields. The normalization protocol eliminates the need for yield quantification prior to analysis. It limits DNA yields uniformly to 150-250 ng.

However, please note that the Normalization protocol should not be used if the DNA amount in the sample is very limited. In this case, the Trace protocol on the EZ1 DNA Investigator Card should be used instead.

FAQ ID -1155
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
Yes, the Tip Dance protocol of the EZ1 DNA Investigator Card can cope with solid matrix that remains in the sample tube. However, very fluffy/diffuse materials (e.g. paper tissues) can clog the tips.
FAQ ID -1153
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
No, the EZ1 DNA Forensic Card cannot be used with the EZ1 DNA Investigator Kit.
FAQ ID -1151
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

For storage of DNA purified from forensic samples using the EZ1 DNA Tissue Kit in a buffered solution such as TE rather than water (used for elution in the EZ1 forensic protocols) we suggest the following:

Before storing the DNA, add 1/10 volume of 10x Forensic TE buffer (100 mM Tris·Cl, 1 mM EDTA, pH 8.3) to each sample. For example, add 5 µl of 10x Forensic TE buffer if you eluted in 50 µl. The DNA is ready to use, or can be stored at 2–8°C for 24 h, or at –20°C for longer periods.

FAQ ID -734
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246
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