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QIAamp MinElute Media Kit

Para la purificación de ADN a partir de medios líquidos

S_1422_RPA_QA0888

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QIAamp MinElute Media Kit

Cat. No. / ID:   57414

Para 50 minipreparaciones: 50 QIAamp MinElute Columns, QIAGEN Proteinase K, ARN transportador, tampones, Extension Tubes (3 ml), Collection Tubes (1,5 ml)
385,00 €
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El QIAamp MinElute Media Kit está concebido para su uso en aplicaciones de biología molecular. Este producto no está concebido para el diagnóstico, la prevención ni el tratamiento de enfermedades.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Purificación a partir de una variedad de medios líquidos de transporte
  • Procedimiento de vacío con ahorro de tiempo para un manejo práctico y facilidad de uso
  • Volúmenes de elución flexibles de entre 20 y 150 µl
  • ADN de gran calidad con eliminación eficiente de alcoholes y contaminantes

Product Details

El QIAamp MinElute Media Kit incluye un procedimiento práctico de vacío para la purificación de ácidos nucleicos a partir de medios líquidos, como los medios de transporte de hisopo para cuello uterino. Las columnas QIAamp MinElute se procesan rápidamente en los QIAvac 24 Plus vacuum manifolds. La purificación de ADN con el QIAamp MinElute Media Kit puede automatizarse en el instrumento QIAcube Connect.

Performance

El QIAamp MinElute Media Kit permite el procesamiento de 250 µl de transporte líquido y muestras de medios de almacenamiento en menos de 90 minutos. El procesamiento de las columnas QIAamp MinElute en QIAvac 24 o QIAvac 24 Plus vacuum manifolds se realiza en lotes de 24 muestras. El ADN se eluye en 20-150 µl.

Principle

El QIAamp MinElute Media Kit emplea tecnología bien establecida para la purificación de ácidos nucleicos. El kit combina las propiedades de unión selectiva de una membrana de gel de sílice con volúmenes de elución flexibles de entre 20 y 150 µl. El kit es adecuado para usarlo con medios líquidos que contienen ácidos nucleicos, como medios de transporte de hisopo para cuello uterino (como la solución PreservCyt o SurePath). Los ácidos nucleicos se eluyen en Buffer AVE, listos para usarse en reacciones de amplificación o almacenarse. Los ácidos nucleicos purificados no contienen proteínas, nucleasas ni otras impurezas.

Procedure

El procedimiento de QIAamp MinElute Media comprende 4 pasos (lisis, unión, lavado y elución) y se lleva a cabo con las columnas QIAamp MinElute en los QIAvac 24 Plus vacuum manifolds. El procedimiento se ha diseñado para garantizar que no haya contaminación cruzada detectable entre muestras y permitir el manejo seguro de muestras potencialmente infecciosas. El sencillo procedimiento de QIAamp MinElute, muy adecuado para el procesamiento simultáneo de varias muestras, produce ácido nucleico puro a partir de 24 muestras en menos de 90 minutos.

Applications

El QIAamp MinElute Media Kit se puede utilizar para aislar ADN a partir de medios líquidos, como los medios de transporte de hisopo para cuello uterino. El kit se puede utilizar para la purificación de ácidos nucleicos celulares, bacterianos y víricos a partir de una variedad de fuentes, entre las que se incluyen las indicadas a continuación:

  • Medios líquidos de citología con alcohol (como PreservCyt y SurePath)
  • Medios líquidos de transporte de tampón fosfato (como M4RT)

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time PCR
FormatColumnas MinElute
Time per run or per prep<90 minutos (24 muestras)
Sample amount250 µl
Elution volume20-150 μl
TechnologyTecnología de sílice
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinADN y ARN víricos, ADN y ARN bacterianos, ADN y ARN celulares
YieldVaría
Main sample typeMedios líquidos
ProcessingManual (vacío)

Resources

Kit Handbooks (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Brochures & Guides (1)
Certificates of Analysis (1)

FAQ

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
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