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PyroMark Buffers and Solutions
For optimal preparation and analysis of template DNA by Pyrosequencing
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PyroMark Denaturation Sol. (500 ml)
Cat. No. / ID: 979007
For denaturation of double-stranded PCR product into single-stranded DNA template
Buffer typeType
Denaturation
Binding
Annealing
Wash
PyroMark Buffers and Solutions are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
Product Details
Generating Pyrosequencing templates from PCR amplicons is fast and straightforward with the PyroMark vacuum workstation. These buffers and solutions are specifically designed for this purpose. PyroMark Binding Buffer facilitates binding of biotinylated PCR amplicons to streptavidin-coated Sepharose beads. These beads are then immobilized with the vacuum tool of the workstation. PyroMark Denaturation Solution gently separates the complementary strand from the biotin-tagged strand destined to become the Pyrosequencing template, and PyroMark Wash Buffer ensures that only the biotin-tagged, single-stranded DNA remains immobilized to the vacuum tool. Finally, when the single-stranded DNA is released into reaction vessels containing the Pyrosequencing primer, hybridization of primer and template is enhanced by PyroMark Annealing Buffer.