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QIAGEN Protease (30 AU)
Cat. No. / ID: 19157
4 x 7.5 Anson units per vial (lyophilized)
EnzymeSolutionAccessories
QIAGEN Protease
QIAGEN Proteinase K
QIAGEN Protease Solvent
Rotary Shaker I
Contains
30 (4 x 7.5) AU
7.5 AU
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- For protease digestion in DNA and RNA isolation procedures
- Fully compatible with selected QIAGEN protocols
- Both enzymes quality-guaranteed by QIAGEN
Product Details
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.
Performance
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.
Protease activity in commonly used buffers*
| Buffer | Activity (%) | |
|---|---|---|
| QIAGEN Protease |
QIAGEN Proteinase K |
|
| 30 mM Tris·Cl | 100 | 100 |
| 30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl† | 210 | 313 |
| 36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl‡ | 205 | 301 |
| 10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS | 78 | 128 |
| 10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl | 101 | 74 |
| 10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100 | 159 | 106 |
| 10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS | 98 | 120 |
| 30 mM Tris·Cl; 10 mM EDTA; 1% SDS | 38 | 203 |
Principle
Technical specifications
| QIAGEN Protease | QIAGEN Proteinase K | |
|---|---|---|
| Format | Lyophilized powder | Ready-to-use solution |
| Amount | 7.5 AU or 4 x 7.5 AU | 2 ml or 10 ml (20 mg/ml) |
| Activity | 45 mAU/mg protein | >600 mAU/ml |
| Unit definition | One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute | |
QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:
- QIAamp DNA Mini Kit
- QIAamp DNA Stool Mini Kit
- QIAamp 96 DNA Swab BioRobot Kit
- DNeasy Tissue Kit
- DNeasy 96 Tissue Kit
- QIAamp DNA Micro Kit
- QIAamp MinElute Media Kit
- QIAamp UltraSens Virus Kit
- QIAamp Media MDx Kit
QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:
- QIAamp DNA Blood Mini, Midi and Maxi Kits
- QIAamp 96 DNA Blood Kit
- Blood & Cell Culture DNA Kits
- QIAamp DNA Blood BioRobot 9604 Kit
- QIAamp Virus BioRobot 9604 Kit
- QIAamp DNA Blood BioRobot MDx Kit
- QIAamp DSP 96 Virus MDx Kit
- QIAamp DSP Virus Kit
- QIAamp DSP DNA Blood Mini Kit
Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.
Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.
Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.
Procedure
Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.
Applications
QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.
Resources
User-Developed Protocols (2)
Safety Data Sheets (6)
Instrument User Manuals (3)
Software User Guides (4)
Supplementary Protocols (2)
Package Insert (1)
FAQ
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?
How can QIAGEN Protease and Proteinase K be inactivated?
3192 - Is QIAGEN Protease compatible with Buffer ATL?