QIAshredder

For simple and rapid homogenization of cell and tissue lysates

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QIAshredder (250)

Cat. No. / ID: 79656

250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps

475,00 US$

Pieces

250

Quantity

El QIAshredder está concebido para su uso en aplicaciones de biología molecular. Este producto no está concebido para el diagnóstico, la prevención ni el tratamiento de enfermedades.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Replaces syringe-and-needle homogenization
Reduces loss of sample material
Eliminates cross-contamination between samples
Filters out insoluble debris and reduces viscosity

Product Details

The QIAshredder homogenizer consists of a unique biopolymer-shredding system in a microcentrifuge spin-column format. Cell or tissue lysate is loaded onto the QIAshredder homogenizer placed in a collection tube and centrifuged. The homogenized lysate is then collected. In general, similar yields and quality of RNA are obtained as with rotor–stator homogenization (see figure "").

See figures

Comparison of RNA yields.

Supporting data and figures

Comparison of RNA yields.

Resources

RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi

Publications

The protective role of a small GTPase RhoE against UVB-induced DNA damage in keratinocytes.

Boswell SA; Ongusaha PP; Nghiem P; Lee SW;

J Biol Chem; 2006; 282 (7):4850-4858 2006 Dec 14 PMID:17170105

Cell specific patterns of methylation in the human placenta.

Grigoriu A; Ferreira JC; Choufani S; Baczyk D; Kingdom J; Weksberg R;

Epigenetics; 2011; 6 (3):368-79 2011 Mar 1 PMID:21131778

Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines.

Abe M; Havre PA; Urasaki Y; Ohnuma K; Morimoto C; Dang LH; Dang NH;

BMC Cancer; 2011; 11 :51 2011 Feb 1 PMID:21284881

Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in MG-63 osteoblast-like cells exposed to polyethylene particles.

Xu J; Kauther MD; Hartl J; Wedemeyer C; Study was performed at the University of Duisburg - Essen, Germany;

J Orthop Surg Res; 2010; 5 :83 2010 Nov 4 PMID:21050480

Alpha-calcitonin gene-related peptide can reverse the catabolic influence of UHMWPE particles on RANKL expression in primary human osteoblasts.

Kauther MD; Xu J; Wedemeyer C;

Int J Biol Sci; 2010; 6 (6):525-36 2010 Sep 15 PMID:20877694

FAQ

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

FAQ ID -1751

The QIAshredder is a unique biopolymer shredding system in a microcentrifuge spin-column format. It homogenizes cell or tissue lysates to reduce viscosity. Homogenization shears the high-molecular weight genomic DNA and other high-molecular-weight cellular components to create a homogenous lysate. The QIAshredder is chemically inert and will not bind nucleic acids.

It cannot replace tissue disruption or enzymatic cell wall lysis by mechanical and chemical methods, respectively. Both, efficient cell wall disruption and lysate homogenization are fundamental for successful RNA isolation from all types of samples. Once complete disruption has been achieved, the QIAshredder Homogenizer can be used in place of needle and syringe, or rotor-stator homogenization.

For further information regarding sample disruption and homogenization please refer to the 'Disruption and homogenization of starting materials' section in the RNeasy Mini Handbook, and see FAQ 139.

FAQ ID -631

The QIAshredder Maxi spin columns from the DNeasy Plant Maxi Kit are not recommended for use in conjunction with QIAGEN's RNA isolation products. The filter can only tolerate low centrifugal forces not sufficient to shear genomic DNA. If genomic DNA is not sheared, there will be an increase in genomic DNA contamination in the purified RNA.

For an alternative to QIAshredder homogenization in combination with RNeasy Midi/Maxi Preps, please see FAQ 560.

FAQ ID -616

We only offer QIAshredder spin columns for use with the RNeasy Mini Kit. When working with RNeasy Midi/Maxi, we recommend using a rotor-stator, or mortar and pestle with needle and syringe homogenization.

FAQ ID -560

Yes

FAQ ID -501

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