qPCR Mixes

• Detects low copies of DNA or cDNA targets
• Produces consistent amplification across a wide dynamic range (6 logs)
• Eliminates non-specific amplification (tested up to 45 cycles)
• Detects molecular targets accurately as fast as 30 minutes
• No loss of activity after eight successive freeze cycles

qPCR SG and Probe Mixes are ready-to-use, 2x concentrated master mix of TaqNova HS polymerase, which is a mixture of recombinant Taq polymerase expressed in E. coli and a particular monoclonal anti-Taq antibody.

The TaqNovaHS polymerase enables easy set-up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures. This prevents the extension of non-specifically annealed primers and primer dimers formed at low temperatures during PCR set-up. The antibody is released from the polymerase during the initial DNA denaturation step, thus providing total DNA polymerase activity during standard cycling conditions.

Precisely optimized buffer components, reliable DNA polymerase and the appropriate selection of PCR enhancers provide a high performance, sensitivity and specificity over a broad dynamic range and give consistent results across all commonly used real-time PCR platforms.

Quality Control

qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of deoxyribonuclease contamination.

This is used for applications such as:

• qPCR or real-time PCR
• Gene expression analysis
• Mass screening and pathogen detection
• Characterization of genetically modified organisms (GMO)