Looking for a quick way to design experiments?
Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs.

dsDNA Mass to Moles Converter

Mass to Moles
Moles to Mass
icon_0330_cc_gen_calculator-s Calculate TargetsEnter the data of the targets you are interested in and receive sequencing calculations
Formula
moles dsDNA (mol) = mass of dsDNA (g)/((length of dsDNA (bp) x 617.96 g/mol/bp) + 36.04 g/mol) moles of dsDNA ends = moles dsDNA (mol) x 2 DNA copy number = moles of dsDNA x 6.022e23 molecules/mol
Read more
moles dsDNA (mol) = mass of dsDNA (g)/((length of dsDNA (bp) x 617.96 g/mol/bp) + 36.04 g/mol)moles of dsDNA ends = moles dsDNA (mol) x 2DNA
          copy number = moles of dsDNA x 6.022e23 molecules/mol

          mass of dsDNA (g) = moles of dsDNA (mol) x ((length of dsDNA (bp) x 617.96 g/mol/bp) + 36.04 g/mol)

          dsDNA Mass to Moles
          moles dsDNA = mass of dsDNA (g)/molecular weight of dsDNA (g/mol)molecular weight of dsDNA = (number of base pairs of dsDNA x average molecular
          weight of a base pair) + 36.04 g/mol average molecular weight of a base pair = 617.96 g/mol, excluding the water molecule removed during polymerization.
          The 36.04 g/mol accounts for the 2 -OH and 2 -H added back to the ends. Bases are assumed to be unmodified. 

          When a sequence is provided, the exact molecular weight is calculated by summing the molecular weights of the individual nucleotides:dA: 313.23 dT: 304.21
          dG: 329.23 dC: 289.20These weights exclude the water molecule removed during polymerization.36.04 g/mol is added for the 2 -OH and 2 -H added back to
          the ends.

          dsDNA Mass to Moles of Ends
          moles of dsDNA ends = moles linear dsDNA (mol) x 2(see above to calculate moles dsDNA from mass)Bases are assumed to be unmodified.

          dsDNA Mass to Molecules
          number of molecules dsDNA = moles of dsDNA x Avagadro's NumberAvagadro's Number = 6.022e23 molecules/mol(see above to calculate moles dsDNA          
          from mass)
icon_0061_360degree-s

Looking for more enzymes?

Read about products that QIAGEN has in its portfolio.

Recommended Items for you

AllTaq PCR Core Kit (250 U)
CAT NO./ID 203123
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
1 050,00 R$
Taq DNA Polymerase (250 U)
CAT NO./ID 201203
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
1 062,00 R$
End-Repair Mix (low concentration)
CAT NO./ID Y9140-LC-L
End-Repair Mix (low concentration)
For 200 low-concentration DNA reactions. End-Repair Mix 1X (0.20 mL), 10X End-Repair Buffer (1 x 1.5 mL) and 1 mM dNTP solution (0.5 mL).
3 325,00 R$
T4 DNA Ligase (Rapid)
CAT NO./ID L6030-HC-L
T4 DNA Ligase (Rapid)
240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
3 905,00 R$
T7 DNA Ligase (900,000 U)
CAT NO./ID L6020L
T7 DNA Ligase (900,000 U)
900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer
2 447,00 R$
Klenow Fragment (2500 U)
CAT NO./ID P7060L
Klenow Fragment (2500 U)
2500 U of Klenow Fragment (0.5 mL at 5000 U/mL) and 10x Blue Buffer (1 x 1.5 mL)
2 447,00 R$
Taq-B DNA Polymerase (10,000 U)
CAT NO./ID P7250L
Taq-B DNA Polymerase (10,000 U)
10,000 U of Taq-B Polymerase (2 mL at 5000 U/mL) and 10x PCR Buffer. 
3 325,00 R$
TaqNova Polymerase UCP (1000 U)
CAT NO./ID RP1010
TaqNova Polymerase UCP (1000 U)
Ultra-Clean Production TaqNova DNA Polymerase. 5 U/µL concentration with reaction buffer of 10x TaqNova DNA-free Reaction Buffer, and 50 mM MgCl2Storage buffer: 20 mM Tris-HCl (pH 8.0 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% (v/v) Nonident P40; 0.5% (v/v) Tween 20; 50% (v/v) glycerol Storage temperature: -20°C
1 825,00 R$
RNase A (17,500 U)
CAT NO./ID 19101
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution) Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
2 430,00 R$
HotStarTaq DNA Polymerase (250 U)
CAT NO./ID 203203
HotStarTaq DNA Polymerase (250 U)
250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
904,00 R$
Omniscript RT Kit (50)
CAT NO./ID 205111
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water
2 182,00 R$
RNase-Free DNase Set (50)
CAT NO./ID 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
1 159,00 R$
QIAGEN Protease (7.5 AU)
CAT NO./ID 19155
QIAGEN Protease (7.5 AU)
7.5 Anson units per vial (lyophilized)
845,00 R$
QIAGEN Proteinase K (2 ml)
CAT NO./ID 19131
QIAGEN Proteinase K (2 ml)
2 ml (>600 mAU/ml, solution)
976,00 R$

Applications & Insights

  1. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  2. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  3. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.

Applications & Insights

  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.

Applications & Insights

Applications & Insights

Applications & Insights

Applications & Insights

Applications & Insights

  1. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  2. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  3. Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.

Applications & Insights

  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.
  • Your scientific journey is unlikely to be easy, but the rewards can be spectacular. Discover the technologies, tools and services you need to help you on your journey.

Applications & Insights

Applications & Insights

Applications & Insights

Applications & Insights

//dev-homepage/applications/digital-pcr/promotions/registration/search/products?query=QIAGEN%20Plasmid%20Mini%20Kit%20(100/search3/products?query=dna/search3/products/dev-search/products?query=dna/dev-search/products/product-categories/discovery-and-translational-research/genomic-services/product-categories/discovery-and-translational-research/genomic-services?disable-wfc=true&disable-dtm=true/products/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/blood-and-cell-culture-dna-mini-kit/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/hifidelity-long-range-and-other-pcr/ucp-hifidelity-pcr-kit/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129056/products/discovery-and-translational-research/lab-essentials/buffers-reagents/maxtract-high-density?catno=129065/products/diagnostics-and-clinical-research/sample-processing/allprep-rnaprotein-kit/BAD_URL/product-categories/discovery-and-translational-research/genomic-services/BAD_URL/products?cmpid=1234&intcmp=456/products?cmpid=asdf&intcmp=qwertysds-searchpromotionsknowledge-and-support/resourcesapplications/enzymes/tools-and-calculatorsapplications/enzymes/tools-and-calculators/ligation-calculator