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BioSprint 96 DNA Blood Kit (48)
Cat. No. / ID: 940054
For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
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For instrument
Biosprint 96
Biosprint 15
Preparations
48
384
Les BioSprint DNA Blood Kits sont destinés aux applications de biologie moléculaire. Ces produits ne sont pas conçus pour le diagnostic, la prévention ou le traitement des maladies.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High-throughput isolation of DNA
- Maximal flexibility to accommodate challenging sample material
- Complete removal of contamination and inhibitors
- No organic extraction or alcohol precipitation
Product Details
BioSprint DNA Blood Kits provide all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots and buccal swabs using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.
Performance
DNA can be successfully purified from a variety of sample types (see table “Typical yields from a range of sample types”).
Typical yields from a range of sample types
| Sample type | Amount of sample | DNA (µg) | |
|---|---|---|---|
| BioSprint 15 | BioSprint 96 | ||
| Muscle, bovine | 25 mg | 16.2 ± 2.5 | 13.5 ± 1.5 |
| Heart, bovine | 25 mg | 5.9 ± 2.6 | 3.4 ± 0.6 |
| Spleen, bovine | 25 mg | 69.1 ± 23.6 | 59.1 ± 4.8 |
| Lung, bovine | 25 mg | 13.8 ± 7.2 | 14.7 ± 5.5 |
| Liver, bovine | 25 mg | 77.8 ± 29.4 | 74.0 ± 22.3 |
| Kidney, bovine | 25 mg | 26.2 ± 18.8 | 33.5 ± 5.4 |
| Ear, sheep | 30 mg | 20.31 ± 1.8 | – |
| Tail, mouse | 1.2 cm (~25 mg) | 32.7 ± 4.6 | 30.9 ± 4.5 |
| HL-60 cultured cells | 2 x 106 cells | 10.1 ± 4.7 | 9.6 ± 5.6 |
|
Blood, human (5–7 x 106 cells per ml) |
200 µl | 4.5–9.0 | 4.5–9.0 |
| Blood, bovine | 200 µl | 6.2–8.0 | 4.4–7.0 |
| Blood, sheep | 200 µl | 5.6–11.2 | 1.2–8.0 |
| Blood, pig | 200 µl | 4.5–9.0 | 4.5–9.0 |
| Blood, dog | 200 µl | 6.7–12.3 | 6.9–16.1 |
| Blood, cat | 100 µl | 4.4–8.3 | 2.9–6.3 |
| Blood, mouse | 100 µl | 2.0–8.0 | 1.5–3.0 |
| Blood, rat | 100 µl | 1.0–4.0 | 1.0–3.0 |
| Blood, bird | 10 µl* | 15.0 ±6.3 | – |
| Blood, fish | 5 µl* | 7.1–10.0 | – |
| Dried blood spots, 903 Specimen Collection Paper | 1 punch (6 mm [1/4 inch] diameter) | 0.30 | 0.10 |
| Dried blood spots, FTA Card | 1 punch (6 mm [1/4 inch] diameter) | 0.12 | 0.05 |
| Buccal swabs | 1 swab | 0.8–2.0 | 0.8–2.0 |
Purified DNA is up to 50 kb in size (see figures " Purification of high-quality genomic DNA from mouse tail" and " Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure " High PCR performance of purified DNA").
See figures
Principle
BioSprint DNA Blood Kits are used with the BioSprint 15 or BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from cells, tissue and blood or blood-related products. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.
See figures
Procedure
Genomic DNA can be purified from cells, tissue and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure " BioSprint DNA procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.
For purification of genomic DNA from tissue, dried blood spots or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with BioSprint DNA Blood Kits). For processing in 96-well format, we recommend sealing S-Blocks with Tape Pads during sample processing (not needed for rapid blood protocol).
See figures
Applications
BioSprint DNA Blood Kits purify DNA for use in a range of downstream applications, including:
- Southern blotting
- PCR and real-time PCR
- Genotyping
Comparison of BioSprint DNA Blood Kits
| Features | BioSprint 15 DNA Blood Kit | BioSprint 96 DNA Blood Kit |
|---|---|---|
| Applications | PCR, real-time PCR, genotyping, blotting | PCR, real-time PCR, genotyping, blotting |
| Elution volume | 200 µl | Custom |
| For automated processing | BioSprint 15 workstation | BioSprint 96 workstation |
| Format | 5-tube strip, S-Block | S-Block, 96-well plate |
| Main sample type | Blood, tissue, cells | Blood, tissue, cells |
| Processing | Automated | Automated |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | DNA | DNA |
| Sample amount | 100 µl/25 mg/2 x 10e6 | 100–250 µl |
| Technology | Magnetic particles | Magnetic particles |
| Time per run or per prep | 45 minutes | 45 minutes |
| Yield | Varies | Varies |
Supporting data and figures
Purification of high-quality genomic DNA from mouse tail.
Mouse tail samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 96 DNA Blood Kit with the BioSprint 96 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (2 µl) from 8 out of 96 samples were visualized by agarose gel electrophoresis. M: markers.
Resources
User-Developed Protocols (1)
Safety Data Sheets (5)
Supplementary Protocols (1)
Kit Handbooks (1)
Quick-Start Protocols (1)
Brochures & Guides (1)
FAQ
Can the BioSprint instruments be placed in a cool room?
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
How do I perform a DNA precipitation to concentrate my sample?
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
How to quantitate DNA purified using the BioSprint 96?
How do I safely inactivate biohazardous flow-through material?
Are the BioSprint 15/96 instruments stand-alone workstations?