TissueRuptor II

For low-throughput sample disruption for molecular analysis

Products

The TissueRuptor II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

TissueRuptor Disposable Probes (25)

Cat. No. / ID: 990890

25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)

402,00 $CA

Quantity

TissueRuptor II (120 V, 60 Hz, US)

Cat. No. / ID: 9002755

Handheld rotor–stator homogenizer, 120 V, 60 Hz (for North America), 5 TissueRuptor Disposable Probes

1 030,00 $CA

Quantity

Features

Rapid, effective disruption of a range of sample types
Disposable probes help to minimize cross-contamination
Time savings through use of disposable probes
Visual monitoring of disruption using transparent probes
Seamless integration with QIAGEN sample technologies

Product Details

The TissueRuptor II is a handheld rotor-stator homogenizer that provides rapid, efficient and flexible disruption of human, animal and plant samples for a wide range of downstream applications. The TissueRuptor II uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.

The TissueRuptor II has all the same features and functionality as the old TissueRuptor.

Performance

QIAGEN has tested use of the TissueRuptor II in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor II ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting and enzyme assays (see figures "" and ""). For most tissues, disruption and homogenization using the TissueRuptor II gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor II allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values," "Reproducible purification of high-quality genomic DNA" and "Efficient disruption and homogenization of animal tissues").

See figures

Successful purification of phosphoproteins.

High performance results in PCR.

Principle

Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. The TissueRuptor II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor II is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAprotect Tissue Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified	Sample type	QIAGEN kit
RNA	Easy-to-lyse tissue	RNeasy Kits
		RNeasy Plus Kits
		RNeasy Protect Kits
RNA	Fiber-rich tissue	RNeasy Fibrous Tissue Kits
RNA	All types of tissue	RNeasy Lipid Tissue Kits
RNA	All types of tissue	RNeasy Universal Tissue Kits
RNA	Plant tissue	RNeasy Plant Mini Kit
RNA	Yeast	RNeasy Kits
RNA	Bacteria	RNeasy Protect Bacteria Kits
microRNA	All types of tissue	miRNeasy Kits
DNA	Human tissue	QIAamp DNA Kits
DNA	Animal tissue	DNeasy Blood & Tissue Kits
Protein	Tissue	Qproteome Mammalian Protein Prep Kit
Phosphoprotein	Tissue	PhosphoProtein Purification Kit
Glycoprotein	Tissue	Qproteome Glycoprotein Kits
DNA and RNA	Tissue	AllPrep DNA/RNA Kits
DNA, RNA, and protein	Tissue	AllPrep DNA/RNA/Protein Mini Kit

Procedure

The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

Applications

The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits.

Supporting data and figures

Reproducible purification of high-quality genomic DNA.

Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.

Efficient disruption and homogenization of animal tissues.

Specifications

Features	Specifications
Disruption principle
Technology
Kits compatible with instrument
Technical data
Typical run time
Features
Throughput

Resources

For low-throughput disruption of biological samples using disposable probes

Download Safety Data Sheets for QIAGEN product components.

FAQ

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

FAQ ID -1751

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.

FAQ ID -374

We recommend using either the TissueRuptor or the TissueLyser for homogenization of tissues stored in Allprotect Tissue Reagent.

Since tissues incubated in Allprotect Tissue Reagent are harder than fresh tissues, longer homogenization times might be necessary (for details, see the Allprotect Tissue Reagent Handbook).

Furthermore, we do not recommend using rotor–stator homogenizers with a metal probe (e.g., Polytron) if proteins will be purified subsequently, as the metal probe can heat the sample.

FAQ ID -1572

Disposable probes can be autoclaved up to 5 times (15 min, 120°C).

FAQ ID -9140

Yes, the stainless steel probe can be autoclaved.

FAQ ID -9141

We do not recommend to autoclave any part of the TissueRuptor, except the probe adapter for disposable probes.

FAQ ID -9142

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143

The minimum volume that should be used is 180 µl in a suitably sized vessel (e.g., 2 ml microcentrifuge tube).

FAQ ID -9144

We do not recommend using the probes more than 5 times. Several options for sterilizing and decontaminating the probes can be found in the TissueRuptor User Manual and TissueRuptor Handbook.

FAQ ID -9146