Products
Les QIAsymphony Virus/Bacteria Kits sont destinés aux applications de biologie moléculaire. Ces produits ne sont pas conçus pour le diagnostic, la prévention ou le traitement des maladies.
Features
- High reproducibility through standardized processing
- Bar codes enable tracking of samples and reagents
- Optimized protocols provide high nucleic acid yields
- Novel, prefilled reagent cartridges for ease of use
Product Details
QIAsymphony Virus/Bacteria Kits provide automated purification of viral nucleic acids and bacterial DNA from a broad range of sample types on the QIAsymphony SP. For example, nucleic acids can be purified from plasma, serum, CSF, respiratory samples, urine, urogenital swabs, and transport media. Kits are available in mini and midi format for sample volumes of 200 μl and up to 1000 μl. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
Performance
QIAsymphony Virus/Bacteria Kits enable robust and reproducible capture of nucleic acids. Purified viral and bacterial nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications. Efficient yields and flexible elution volumes (60 μl, 85 μl, or 110 μl) to suit the requirements of downstream applications ensure sensitive analytical detection (see figure " Sensitive detection of Chlamydia trachomatis in urine").
See figures
Principle
QIAsymphony Virus/Bacteria Kits combine the speed and efficiency of silica-based purification of viral and bacterial nucleic acids with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures
Procedure
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
| Protocol | Kit | Sample material | Processed volume |
|---|---|---|---|
| Cellfree 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | Plasma, serum, and CSF | 200 μl |
| Cellfree 500 | QIAsymphony Virus/Bacteria Midi Kit (96) | Plasma, serum, and CSF | 500 μl |
| Cellfree 1000 | QIAsymphony Virus/Bacteria Midi Kit (96) | Plasma, serum, and CSF | 1000 μl |
| Complex 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 200 μl |
| Complex 400 | QIAsymphony Virus/Bacteria Midi Kit (96) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 400 μl |
| Complex 800 | QIAsymphony Virus/Bacteria Midi Kit (96) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 800 μl |
Applications
QIAsymphony Virus/Bacteria Kits enable highly sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.
Supporting data and figures
Sensitive detection of Chlamydia trachomatis in urine.
DNA was purified from 800 μl urine with elution in 110 μl. Bacterial DNA was detected using a commercially available assay amplifying a specific sequence of the C. trachomatis genome on an ABI PRISM 7900HT SDS.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR |
| Elution volume | 60-110 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Purification of viral RNA and DNA and bacterial DNA |
| Input volume | 800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol) |
| Technology | Magnetic particles |
| Sample types | Serum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens) |
Resources
Safety Data Sheets (3)
Labware Files and Documents (2)
Kit Handbooks (1)
FAQ
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?