DNeasy PowerSoil Pro Kits

For the isolation of microbial genomic DNA from all soil types

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DNeasy PowerSoil Pro Kit (50)

Cat. No. / ID: 47014

For the isolation of microbial genomic DNA from all soil types.

449,00 €

Kit

DNeasy PowerSoil Pro Kit

DNeasy PowerSoil Pro QIAcube Kit

Preparations

250

Quantity

Les DNeasy PowerSoil Pro Kits sont destinés aux applications de biologie moléculaire. Ces produits ne sont pas conçus pour le diagnostic, la prévention ou le traitement des maladies.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Efficient lysis of bacteria and fungi in all soil types
Up to 8-fold higher DNA yields compared to alternative methods
Recovery of inhibitor-free DNA for direct use in NGS applications
Unbiased results accurately represent sample alpha diversity
Automation on the QIAcube Connect

Product Details

Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types, including compost, clay and top soil. The kit features a novel bead tube and optimized chemistry for more efficient lysis of soil bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate the challenging inhibitors commonly found in soil and environmental samples in even less time. Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods. Extraction of DNA using the DNeasy PowerSoil Pro Kit can be automated on the QIAcube Connect.

Want to try this solution for the first time? Request a quote for a trial kit.

Performance

Significantly increased DNA yields from challenging samples

The DNeasy PowerSoil Pro Kit includes a novel bead tube and improved lysis chemistry, which enables isolation of up to 8-fold higher yields of DNA compared to the first generation DNeasy PowerSoil Kit and those of competitors in all soil types tested (see Isolate more high-quality genomic DNA). Both the DNeasy PowerSoil Kit and the new DNeasy PowerSoil Pro Kit isolated high-quality DNA from challenging soil types (see Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit).

Unbiased identification of total diversity and community representation in soil samples

DNA isolated using the DNeasy PowerSoil Pro Kit identified more bacteria (OTUs) in soil samples when compared with the DNeasy PowerSoil Kit and those of two competitors (see Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit).

See figures

Figure 1: Isolate more high-quality genomic DNA.

Figure 2: Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.

Figure 3: Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.

Principle

Inhibitor Removal Technology increases purity of isolated DNA

The DNeasy PowerSoil Pro Kit features a streamlined Inhibitor Removal Technology (IRT) to decrease sample processing time. In a comparison of methods for DNA isolation from soil, the DNeasy PowerSoil Pro Kit was the only method showing 260/280 ratios near 1.8 for all soil types, as well as the highest 260/230 ratios, indicating the absence of inhibitors. Samples isolated using the kit also showed no inhibition in PCR, in contrast to competitor methods (see Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit).

See figures

Figure 4: Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.

Procedure

The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or the high-powered PowerLyser. Crude lysate is then subjected to inhibitor removal for cleanup. Following the cleanup, purified lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is washed with a two-step washing regime. Silica-bound DNA is then eluted using a 10 mM Tris elution buffer. Relevant downstream applications for the isolated DNA include PCR, NGS and enzymatic digestion assays.

Supporting data and figures

Figure 1: Isolate more high-quality genomic DNA.

250 mg of the three different soil types were prepared using commercially available sample preparation solutions and compared to the new DNeasy PowerSoil Pro Kit. The DNeasy PowerSoil Pro Kit out-performed other available kits, resulting in 8-fold greater recovery of DNA across the variety of soil types.