TissueLyser Adapter Sets

TissueLyser Adapter Set 2 x 96 should be used with Collection Microtubes (racked) and Collection Microtube Caps (cat. nos. 19560 and 19566 respectively).

Yes, we have the following protocols:

• Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
• Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
• Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).

We do not recommend using the adapters of the TissueLyser II with liquid nitrogen. Instead, the adapters can be precooled at –80°C, either in the freezer or on dry ice.

100 mg tissue per sample tube is the maximum amount we recommend when using the 2 x 24 Adapter Set. Larger amounts of tissue would require considered optimization, depending on the tissue type and condition.

When using 100 mg tissue, for best homogenization results, we recommend cutting the sample into 2 pieces of approximately 50 mg each prior to disruption and performing disruption twice, each time at 25 Hz for 5 min.