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GeneRead Size Selection Kit (50)
Cat. No. / ID: 180514
For 50 reactions: Spin columns and buffers
Le GeneRead Size Selection Kit est destiné aux applications de biologie moléculaire. Ce produit n’est pas conçu pour le diagnostic, la prévention ou le traitement des maladies.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Precise size selection of DNA fragments
- Fast procedure, based on QIAGEN’s proven silica-column technology
- An easy-to-follow protocol, automatable on the QIAcube
Product Details
The GeneRead Size Selection Kit enables precise size selection and purification of DNA fragments using a fast and convenient spin-column-based procedure. The kit contains MinElute columns and an optimized binding buffer for the selection of DNA fragments larger than 150 bp. Procedures can be automated on the QIAcube Connect.
Performance
Fast and precise size selection
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers (see figure Precise size selection). DNA fragments shorter than 150 bp are reliably removed.
See figures
Principle
The GeneRead Size Selection Kit combines the convenience of spin-column technology with the selective properties of a uniquely designed binding buffer. MinElute spin columns provided with the kit ensure high concentrations of purified DNA for subsequent reactions. The special buffer provided with the kit is optimized for efficient removal of small DNA fragments such as adapters and adapter dimers. Larger DNA fragments adsorb to the silica membrane in the presence of optimized concentrations of salt, while contaminants and smaller fragments pass through the column. This leads to a cut-off limit (defined as the length of the shortest fragment that can be visualized by electropherogram) of 150 bp. Impurities such as enzymes from previous reactions are efficiently washed away, and pure and size-selected DNA is eluted. For optimal adapter monomer and dimer removal, together with high DNA recovery, two subsequent purification steps are performed for size selection during library preparations for next-generation sequencing.
Procedure
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers. Automatable on the QIAcube, the easy-to-use protocol saves time by eliminating tedious handling procedures, and ensures there is no risk of carryover of potentially inhibitory ethanol or beads (see figure Precise size selection).
See figures
Applications
The GeneRead Size Selection Kit enables quick and reliable removal of DNA fragments shorter than 150 bp for subsequent use in library preparation in NGS.
Supporting data and figures
Precise size selection.
The GeneRead Size Selection Kit effectively removes adapter dimers and adapter monomers following library preparation. A scaled-up image of the above data showing the correct size distribution of Illumina-compatible library fragments following size selection is shown in inset. FU: Fluorescence units.
Resources
Brochures & Guides (3)
Safety Data Sheets (2)
Supplementary Protocols (1)
Additional Resources (1)
Kit Handbooks (2)
Protocol Files (1)
FAQ
I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?