QIAsymphony RNA Kit

For automated purification of total RNA, or total RNA with miRNA, from tissues or cells

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QIAsymphony RNA Kit

Cat. No. / ID: 931636

For as many as 192 preps, depending on protocol: includes 2 reagent cartridges and enzyme racks and accessories

Le QIAsymphony RNA Kit est destiné aux applications de biologie moléculaire. Ce produit n’est pas conçu pour le diagnostic, la prévention ou le traitement des maladies.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Prefilled reagent cartridges for ease of use
High reproducibility through standardized processing
Optimized protocols provide high yields of pure RNA
Bar codes enable tracking of samples and reagents

Product Details

The QIAsymphony RNA Kit enables automated purification of total RNA, or total RNA including miRNA and other small RNAs, from 1–96 samples on the QIAsymphony SP. Total RNA can be purified from animal cells, tissues, or FFPE tissues, using very small amounts of starting material (≥100 cells) up to 1 x 10⁷ cells or 50 mg of tissue. Optimized protocols provide high yields of pure total RNA, or total RNA including miRNA, and flexible elution volumes allow RNA concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Performance

Automated purification of RNA on the QIAsymphony SP is highly reproducible and yields of high-quality RNA from cultured cells, tissues, and FFPE tissues are comparable to those obtained using a manual purification procedure (see figure ""). The QIAsymphony protocols for total RNA purification primarily enrich for RNA molecules longer than 200 nucleotides, including mRNA (see figure ""). The QIAsymphony miRNA protocol also purifies total RNA but does not exclude RNAs <200 nucleotides such as miRNAs and other small RNAs (see figure "").

See figures

Reproducible purification of RNA.

Highly pure intact RNA.

Automated purification comparable to manual procedure.

Principle

The QIAsymphony RNA Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles (see figure "") for purification of total RNA >200 nucleotides, or total RNA including miRNA and other small RNAs after proteinase K treatment. Starting material includes animal cells and tissues. With some additional components, formalin-fixed paraffin-embedded (FFPE) tissues can also be processed with the kit. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

See figures

Efficient handling of magnetic particles.

Procedure

Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes. The cartridges are automatically opened by the QIAsymphony SP helping to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control. The purification procedure comprises 4 steps: lyse, bind, wash, and elute. A number of protocols is available for the QIAsymphony SP for purifying RNA, depending on the starting material and the required RNA product (see table).

Protocols for purification of RNA with the QIAsymphony SP instrument
Protocol	Running with	Sample material (Pretreated sample lysates)	Processed volume (μl)
RNA CT 400	QIAsymphony RNA Kit (2 x 96)^‡	≤3 x 10⁶ cultured cells ≤20 mg easy-to-lyse tissue	400 μl
RNA CT 800	QIAsymphony RNA Kit (2 x 48)^‡	3 x 10⁶–1 x 10⁷ cultured cells ≤50 mg easy-to-lyse tissue	800 μl
RNA FT 400*	QIAsymphony RNA Kit (2 x 96)^‡	≤20 mg fibrous tissue	400 μl
miRNA CT 400*	QIAsymphony RNA Kit (2 x 96)^‡	≤3 x 10⁶ cultured cells ≤20 mg tissue	400 μl
RNA FFPE 130^†	QIAsymphony RNA Kit (2 x 72)^‡	1–2 x FFPE sections up to 10 μm 1 x FFPE section 20 μm	130 μl

* Proteinase K required; can be ordered separately.
^† Additional components required; can be ordered separately.
^‡ Values in parentheses indicate maximum number of samples that can be processed with kit/protocol combination.

See figures

Prefilled, sealed reagent cartridges.

Applications

The QIAsymphony RNA Kit, and fully automated purification of total RNA on the QIAsymphony SP, provides high-quality RNA suitable for direct use in any downstream application.

Supporting data and figures

Reproducible purification of RNA.

RNA was purified from Jurkat cells on the QIAsymphony SP using the QIAsymphony RNA Kit. RNA yields from 2 instrument runs using 2 different reagent cartridge lots were compared with manual purification using the RNeasy Mini Kit. Each bar represents the average of 4 samples.

Specifications

Features	Specifications
Applications	RT-PCR; Real-Time PCR; Expression Profiling
Sample amount	400 µl lysate from <20 mg tissue or 3 x 10e6 cells or 800 µl lysate from <50 mg tissue or 3 x 10e6 cells
Elution volume	50-200 µl

Resources

Download Safety Data Sheets for QIAGEN product components.

For use with software version 4.0

For use with software version 4.0 or higher

For use with software version 4.0

For use with software version 5.0 or higher

For use with the QIAsymphony SP/AS instruments (software version 4.0)

FAQ

RNA processed on the QIAsymphony SP with the QIAsymphony RNA Kit can be eluted in 200, 100, or 50 µl.

Elution in smaller volumes will provide higher concentration, but lower overall yield. Total RNA yield with elution in 50 µl will be about 20% lower compared to elution in 100 µl.

FAQ ID -1943

Using the Standard protocol for RNA extraction on the QIAsymphony SP, up to 400 µl of lysate can be processed while the Large-Volume protocol allows up to 800 µl of homogenate.

FAQ ID -1939

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

FAQ ID -1904

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

FAQ ID -1906

For purification of RNA from blood on the QIAsymphony SP, we recommend using the QIAsymphony PAXgene RNA Kit, since it includes stabilization of gene expression profiles.

FAQ ID -1945

For purification of RNA on the QIAsymphony SP, tissue samples require mechanical disruption and homogenization in the supplied lysis buffer (Buffer RLT Plus), e.g., by using the TissueLyser or TissueRuptor. Cultured cells should be lysed by vortexing in the lysis buffer contained in the kit.

FAQ ID -1938

200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.

FAQ ID -1907

Yes, the QIAsymphony RNA Kit is compatible with stabilized samples. We recommend stabilizing tissue samples with RNAprotect Tissue Reagent or AllProtect Tissue Reagent, or by flash-freezing. Cultured cells can be lysed directly in cell culture dishes, or after pelleting. Alternatively, cells can be stabilized using RNAprotect Cell Reagent.

FAQ ID -1944

The QIAsymphony RNA Kit enables purification of RNA from cultured cells and easy-to-lyse tissues, such as kidney, liver, and spleen on the QIAsymphony SP. A large-volume protocol is available that enables processing of larger sample amounts.

Cultured cells: up to 3x10⁶ cells (standard protocol); up to 1x10⁷ (large volume protocol)
Tissues: up to 20 mg of most tissues (standard protocol); up to 50 mg (large-volume protocol)

For particularly DNA-rich tissues, such as spleen or thymus, we recommend either using no more than half the maximum amount, or using DNase at a higher concentration (see the QIAsymphony RNA Handbook; additional DNase required).

FAQ ID -1937

Strongly denaturing conditions in the lysis buffer (Buffer RLT Plus) of the QIAsymphony RNA Kit keep RNases in an inactive state. Keeping homogenates on the QIAsymphony instrument at room temperature (20-25°C) for over 6 hours does not impair RNA integrity.

FAQ ID -1941

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

FAQ ID -1903

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

FAQ ID -1908

Please see expected yields of total RNA isolated with the QIAsymphony RNA Kit on the QIAsymphony SP in the table below:

Sample type	Sample amount	Typical RNA yield (µg)*
Cultured Cells
HeLa, Jurkat	1 x 10e6 cells	15
Rat tissues
Kidney	10 mg	10-25
Liver	10 mg	20-50
Lung	10 mg	5-10
Spleen	10 mg	25-35

*RNA yields strongly depend on the type and quality of the sample material.

FAQ ID -1942

Each QIAsymphony RNA Kit enables preparation of up to 192 samples (96 per reagent cartridge) using the standard protocol on the QIAsymphony SP. If only the large-volume protocol is used, the number of samples that can be prepared per kit is reduced to 96 (48 per reagent cartridge). Any combination of standard and large-volume sample preparation can be performed.

FAQ ID -1940