QIAamp Virus BioRobot 9604 Kit

• Rapid isolation of high-quality, ready-to-use viral DNA and RNA
• No organic extraction or alcohol precipitation
• Consistent, high yields
• Removal of contaminants and inhibitors

The QIAamp Virus BioRobot 9604 Kit provides automated viral nucleic acid purification on the BioRobot 9604 using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours for up to 96 samples, including bar code reading and complete process documentation, with no hands-on time during the run. 

QIAamp 96 plates provide well-to-well uniformity in RNA and DNA recovery for reliable results in downstream applications. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in efficient wash steps, leaving pure nucleic acids to be eluted in a buffer provided with the kit.

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acid is then eluted in a low-salt buffer using the BioRobot 9604 (see flowchart " Protocol").

Highly pure, viral RNA and DNA can be isolated from 96 cell-free fluid samples in less than 2.5 hours. This kit can be used to isolate nucleic acids from human and animal viruses. QIAamp sample preparation technology is fully licensed.

The QIAamp Virus BioRobot 9604 Kit provides proven QIAamp technology in a convenient 96-well format for automated high-throughput purification of viral nucleic acids on the BioRobot 9604. The kit provides purification of nucleic acids from 200 µl or less of samples, including:

• Plasma
• Serum
• CSF
• Other cell-free body fluids
• Cell-culture supernatants