artus Parvo B19 PCR Kits RUO

• High reliability using the internal control
• Sensitive detection of as few as 0.2 IU/µl (ABI PRISM 7000)
• Accurate quantitation using the 5 standards supplied

To ensure sensitivity, artus Parvo B19 PCR Kits have been optimized to detect low numbers of parvovirus B19 DNA. The analytical sensitivity of the artus Parvo B19 TM PCR Kit is 0.2 IU/µl in the PCR on the ABI PRISM 7000 SDS.

artus Parvo B19 PCR Kits are based on the amplification and simultaneous detection of a specific region of the parvovirus B19 genome using real-time PCR. The kits provide high levels of specificity and sensitivity.

Each artus Parvo B19 PCR Kit provides 5 quantitation standards (see figure " Reliable quantitation of parvovirus B19 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical parvovirus B19 PCR is not reduced.

artus Parvo B19 PCR Kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix (and Mg solution, LC kit only), and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, we recommend using the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit.