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EZ1 DSP DNA Blood Kit (48)
Cat. No. / ID: 62124
Pour 48 préparations d’ADN : Cartouches de réactif préremplies, porte-pointes jetables, pointes de filtres jetables, tubes d’échantillon, tubes d’élution
The EZ1 DSP DNA Blood Kit is intended for in vitro diagnostic use.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- For in vitro diagnostic use
- Rapid purification of high-quality DNA from whole blood samples
- Straightforward and easy automated purification on the EZ2 Connect MDx
- Standardized processing ensures reproducible results
Product Details
The EZ1 DSP DNA Blood Kit contains all required reagents and labware for automated purification of DNA from 200 or 350 μl human whole blood using magnetic-particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the EZ1 Advanced or the EZ1 Advanced XL or the EZ2 Connect MDx. The CE-IVD-marked system provides exact performance specifications, assuring highly reliable DNA purification.
Performance
Automated DNA purification provides high levels of reliability and standardization and helps to eliminate variation caused by handling errors. The EZ1 Advanced XL or the EZ2 Connect MDx in combination with the EZ1 DSP DNA Blood Kit enables efficient purification of genomic DNA from human whole blood.
Principle
Automated sample preparation enables efficient, standardized purification of nucleic acids. The EZ2 Connect MDx and the EZ1 Advanced XL enable purification of nucleic acids using proven magnetic-particle technology for use in routine in vitro diagnostic applications. Reagent production is subjected to stringent quality control assuring reliable and robust performance.
An easy-to-use instrument, prefilled and sealed reagent cartridges, and simple protocol selection and worktable setup help to prevent handling errors and allow the system to be operated by anyone — from the novice to the expert. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. Purified genomic DNA is ready to use in sensitive downstream diagnostic assays, such as PCR.
Procedure
The EZ2 Connect MDx or the EZ1 Advanced XL in combination with the proven EZ1 DSP DNA Blood Kit enables purification of DNA from1–24 samples (EZ2 Connect MDx) or 1-14 samples (EZ1 Advanced XL) of human whole blood. All processing steps are performed by the instrument, from piercing the reagent cartridge to elution of highly pure nucleic acids. The innovative design of the instrument means that the pipet tips function as separation chambers, increasing the efficiency of magnetic purification and eliminating the need for centrifugation steps. The streamlined procedure provides robust walkaway purification of nucleic acids with minimal hands-on time.
Applications
With the EZ2 Connect MDx or the combination of the EZ1 Advanced XL DSP DNA Blood Card and the EZ1 Advanced XL, the EZ1 DSP DNA Blood Kit provides a fully automated procedure for purification of DNA from human whole blood. Genomic DNA purified using the EZ1 DSP DNA Blood Kit is ready to use in downstream diagnostic assays.
Specifications
| Features | Specifications |
|---|---|
| Elution volume | 50–200 µl |
| Applications | Downstream detection procedures in molecular diagnostics, such as PCR |
| Sample amount | 200 µl or 350 µl |
| Technology | Magnetic particles |
| Main sample type | Whole blood |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| CE/FDA/IVD compatible | CE/IVD |
| For automated processing | EZ2 Connect MDx and EZ1 Advanced XL |
| Yield | Varies on WBC count, please see handbook |
| Processing | Automated |
Resources
Safety Data Sheets (2)
Performance Data (1)
Kit Handbooks (4)
Application Notes (6)
FAQ
How long can eluates be stored after sample preparation using the BioRobot EZ1 DSP instrument?
What do I have to do if precipitates are visible in the lysis buffer (well 1 of the reagent cartridge [RCB])?
Can samples other than whole blood be used with the EZ1 DSP DNA Blood Kit?
How long can blood samples be stored before sample preparation using the EZ1 DSP DNA Blood system?
Can I use sample preparation cartridges from other suppliers together with the BioRobot EZ1 DSP instrument?
How can I track samples during collection, sample preparation, and downstream assay?