At a glance
The Q-Rex operating and analysis software provides unique features to facilitate fast analysis of your experiments on the QIAquant and Rotor-Gene Q real-time PCR instruments.
It enables experiment setup and data acquisition and performs basic analyses. In addition, you can expand Q-Rex functionality with plug-ins for specific capabilities such as absolute quantification and genotype analysis.
The software is suitable for use by operators new to real-time PCR while maintaining the capacity for highly complex data analysis required for advanced research.
Top 7 benefits of the Q-Rex software
Introducing the instruments
Expand the capacity of Q-Rex software with plug-ins
Plug-ins for Q-Rex software
The core software of Q-Rex enables experiment setup and data acquisition, while the plug-ins expand the capacity of Q-Rex with specific analytical capabilities. It allows you to easily create the exact software configuration to meet your specific analysis needs without installing functions you will never use. Let’s take a closer look at the plug-ins and what they can do:
Q-Rex Basic Plug-in
- Suitable for instrument users getting started with general qPCR and RT-PCR
- Drives the hardware, sets up qPCR, melt analyses and multiplexing experiments
- Collects raw data and performs basic Cq analysis
Q-Rex Gene Expression Advanced Plug-in
- Analyzes changes in gene expression relative to a reference sample
- Allows multiple reference gene normalization based on algorithms recommended in industry-leading literature
- Defines a stable reference gene with the help of plots
- Allows normalization of values in relation to a defined calibrator sample
- Defines the real qPCR efficiency for accurate results
Q-Rex Absolute Quantification Plug-in
- Calculates the absolute concentration of targets based on a standard curve
- Applications include:
- Quantification of NGS libraries
- Quantification for exosome enrichment
- Quantification for assessing genome structure like telomeres
Q-Rex End Point Analysis Plug-in
- Can be used for probe-based SNP genotyping
- Automates genotype calling based on advanced algorithms for easy and convenient genotype analysis
- Delivers both raw and normalized data analysis
Q-Rex Melt Curve Analysis Plug-in
- Performs advanced analysis that can be applied for genotyping or multiplex pathogen detection of >15 targets
- New data processing algorithms for easy and improved melt peak detection
- High multiplex performance comes from use of TaqMelt™ technology and powerful algorithms that improve analysis and enable combination potential
Q-Rex Target Detection Plug-in
- Versatile; detects the presence or absence of targets based on user-defined criteria
- Delivers results as an intuitive stoplight visualization
- For the Rotor-Gene Q
- Includes pre-defined assay rules for several QIAGEN Assays
- Accommodates multiplexing with FRET probes
- For the QIAquant
- Users can design lab-developed assays with adapted rules
Q-Rex Absolute Quantification Plug-in HID (only for RGQ)
- Calculates the absolute concentration of targets in experimental samples using the amplification curves of samples of known concentration to establish a standard
- Has an automatic threshold function based on the defined standards that scans through all possible threshold levels until the best fit is found for the standard curve
Q-Rex High Resolution Melt Plug-in (only for RGQ)
- Simplifies high-resolution melting analysis to detect small sequence differences
- Performs automated genotype calling based on standards
- Calculates confidence levels for all results
- Results are reported on difference plots for meaningful and clear visualization
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