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QIAamp MinElute Media Kit
Cat. No. / ID: 57414
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Proteinase K, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)
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The QIAamp MinElute Media Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Purification from a variety of liquid transport media
- Time-saving vacuum procedure for convenient handling and ease of use
- Flexible elution volumes from 20 to 150 µl
- High-quality DNA with efficient removal of alcohols/contaminants
Product Details
The QIAamp MinElute Media Kit provides a convenient vacuum procedure for purification of nucleic acids from liquid media, such as cervical swab transport media. QIAamp MinElute columns are rapidly processed on QIAvac 24 Plus vacuum manifolds. Purification of DNA using the QIAamp MinElute Media Kit can be automated on the QIAcube Connect.
Performance
QIAamp MinElute Media Kit allows 250 µl liquid transport and storage media samples to be processed in <90 minutes. QIAamp MinElute columns processed on QIAvac 24 or QIAvac 24 Plus vacuum manifolds are processed in batches of 24 samples. DNA is eluted in 20–150 µl.
Principle
The QIAamp MinElute Media Kit uses well-established technology for purification of nucleic acids. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The kit is suitable for use with liquid media containing nucleic acids, such as cervical swab transport media (e.g., PreservCyt or SurePath solution). Nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Procedure
The QIAamp MinElute Media procedure comprises 4 steps (lyse, bind, wash, elute) and is carried out using QIAamp MinElute columns on QIAvac 24 Plus vacuum manifolds. The procedure is designed to ensure that there is no detectable sample-to-sample cross-contamination, and allows safe handling of potentially infectious samples. The simple QIAamp MinElute procedure, which is highly suited for simultaneous processing of multiple samples, yields pure nucleic acid from 24 samples in less than 90 minutes.
Applications
The QIAamp MinElute Media Kit can be used for isolation of DNA from liquid media, such as cervical swab transport media. The kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including:
- Liquid cytology media with alcohol (e.g., PreservCyt and SurePath)
- Phosphate-buffered liquid transport media (e.g., M4RT)
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR |
| Format | MinElute columns |
| Time per run or per prep | <90 minutes (24 samples) |
| Sample amount | 250 µl |
| Elution volume | 20–150 µl |
| Technology | Silica technology |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA |
| Yield | Varies |
| Main sample type | Liquid media |
| Processing | Manual (vacuum) |
Resources
Kit Handbooks (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Brochures & Guides (1)
Certificates of Analysis (1)
FAQ
I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?