TransMessenger Transfection Reagent
高效的mRNA转染
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TransMessenger Transfection Reagent (0.5 ml)
Cat. No. / ID: 301525
For 60 transfections in 6-well plates or 80 transfections in 12-well plates
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TransMessenger Transfection Reagent 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
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✓ 博学专业的产品和技术支持
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特点
- 高效的转染
- 严格的质量监控确保可靠的结果
- 高效转染原代细胞
产品详情
TransMessenger Transfection Reagent是即用型、脂质体试剂，用于真核细胞的RNA转染。使用RNA而非DNA转染的细胞为转染实验提供了新的可能性。使用TransMessenger Reagent成功转染的细胞株包含293T、Jurkat细胞和Vero细胞。该产品也可用于RNA转染人类原代成纤维细胞、大鼠心肌细胞和大鼠神经细胞。
绩效
TransMessenger Reagent提供快速简单的操作流程和高转染效率（参见" TransMessenger Reagent with HeLa cells"）。因为RNA量是成功转染的关键因素，我们推荐对于每个细胞类型和RNA，都优化RNA和TransMessenger Transfection Reagent量（参见" Amount of RNA and TransMessenger Reagent vs. transfection efficiency"）。为了操作便利，该试剂配套提供优化准则和在不同细胞培养物规格下的优化起始点。
查看图表
原理
TransMessenger Transfection Reagent是首款专为用RNA转染细胞的试剂。该试剂由脂质体制备，与特定的RNA冷凝增强剂和优化的缓冲液一起使用。RNA分子通过增强剂冷凝，然后涂上TransMessenger Reagent有效地转移到真核细胞中。严格的质量监控用于测试无RNA酶活性、每批次的一致性和低内毒素水平（≤10 EU/ml）。我们严格的标准消除了试剂的变量，避免对RNA转染效率产生不利影响。
程序
TransMessenger Reagent为即用型试剂。为获得TransMessenger–RNA转染混合物，只需简单混合具增强剂R的RNA和Buffer EC-R，并在室温下孵育5分钟，然后加入TransMessenger Reagent再孵育5–10分钟。将其与培养液混合并直接加入细胞中。随后需要3小时的孵育，培养液发生改变，孵育细胞直到可用于分析应用。使用高纯度的RNA可获得最佳的转染结果，无DNA、蛋白质和其他污染物。推荐使用RNeasy和Oligotex mRNA Kits纯化RNA。
应用
RNA而非DNA转染的细胞为转染实验提供新的可能性。转染RNA序列的表达无需转录、不依赖于启动子。此外，蛋白表达通常会在RNA而非DNA转染后快速出现。使用TransMessenger Transfection Reagent的RNA转染可用于：
- 研究无法用质粒DNA高效转染的细胞
- 直接研究RNA功能
辅助数据和图表
Amount of RNA and TransMessenger Reagent vs. transfection efficiency.
Optimization experiments were performed in CHO-K1 cells. Cells (2 x 104) were seeded in quadruplicate into 96-well plates and transfected 24 hours later with an in vitro-transcribed CAT-encoding RNA using [A] increasing amounts of RNA with 1.5 µl TransMessenger Reagent and [B] increasing amounts of TransMessenger Reagent with 0.25 µg RNA, as described in the TransMessenger Transfection Reagent Handbook. CAT activity was measured 24 hours post-transfection.
Specifications
| Features | Specifications |
|---|---|
| Applications | Direct studies of RNA function |
| Features | Transfection with RNA. Efficient transfection of neuronal cells. Tested for the absence of RNases. |
| Controls | Not included |
| Nucleic acid | RNA |
| Cell type | Eukaryotic cells |
| Number of possible transfections | 80 transfections in 12-well plates / 0,5 ml reagent |
| Technology | Lipid-based formulation in conjunction with a RNA-condensing enhancer |
| Transfection type | Transient transfection, co-transfection |
资源
转染实验方案 (2)
试剂盒操作手册 (1)
产品介绍与指南 (3)
快速启动实验方案 (1)
安全数据表 (1)
补充实验方案 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Do you have transfection data for QIAGEN Transfection Reagents?
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
What is the recipe for 1x PBS solution?
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?