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QIAsymphony DSP Virus/Pathogen Kit

ウイルス核酸あるいはバクテリアDNA の自動精製

Products

The QIAsymphony DSP Virus/Pathogen Kits are intended for in-vitro diagnostic use.
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QIAsymphony DSP Virus/Pathogen Midi Kit

Cat. No. / ID:   937055

For 96 preps (1000 µl each): includes 2 reagent cartridges and enzyme racks and accessories
€978.00
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QIAsymphony DSP Virus/Pathogen Mini Kit

Cat. No. / ID:   937036

For 192 preps (200 µl each): includes 2 reagent cartridges and enzyme racks and accessories
€1,414.00

特徴

  • 標準化された調製法で高い再現性
  • バーコードによりサンプルや試薬の使用履歴トラッキングが可能
  • 至適化済みのプロトコールで高純度核酸を高収量で精製
  • 使いやすい斬新な充填済み試薬カートリッジ

製品詳細

QIAsymphony DSP Virus/Pathogen Kits とQIAsymphony SP の組み合わせは、多様な検体からウイルス核酸およびバクテリア/病原体由来DNA の自動精製を実現します。キットは200 μl あるいは最大1,000 μl までの検体量に応じて、それぞれMini、Midi のフォーマットがあります。至適化済みのプロトコールにより高純度DNA が高収量で得られ、溶出容量が調節可能なのでダウンストリームアプリケーションごとにDNA 濃度の至適化が簡単にできます。斬新で充填済みの試薬カートリッジによりセットアップ時間を短縮し、マニュアルでの取り扱いは最小限に抑えられます。バーコードによる読み取りにより、試薬のトラッキングを完全に行なえます。

原理

QIAsymphony DSP Virus/Pathogen Kitsは、血清、血漿、CSFからウイルス核酸を精製するために使用するだけではなく、尿や泌尿生殖器のスワブ(子宮頸管や尿道)同様、スワブ、吸引液、気管支肺胞洗浄(BAL)のような呼吸器系検体からウイルス核酸およびバクテリアDNAを精製できます。本キットは、様々なDNAおよびRNAウイルスからの核酸精製またはグラム陰性菌やグラム陽性菌からのバクテリアDNAの精製に用いることができます。

QIAsymphony DSP Virus/Pathogen Kitsは、シリカゲルベースによる効率的で迅速なウイルス/バクテリア核酸精製と磁性粒子による簡便操作を組み合わせています(図“ QIAsymphony SP原理の模式図”)。革新的で即使用可能な試薬カートリッジは、精製工程に必要なすべての試薬が充填済みです。 QIAsymphony SPはカートリッジを自動的に開封し、実験環境からのコンタミのリスクを回避します(図" 充填済みのプレパック試薬カートリッジ”)。至適化済みのプロトコールにより、タンパク質、ヌクレアーゼ、その他の不純物を効率的に除去し、高品質核酸を精製します。QIAsymphony DSPVirus/Pathogen Kitsで精製した核酸はダウンストリームのアプリケーション(PCRなど)ですぐに使用できます。ユーザーは適切な溶出量を選択できます。

図参照

操作手順

精製操作は感染性の高いサンプルを安全かつ再現性よく取り扱えるようにデザインされ、溶解、結合、洗浄、溶出の4ステップで構成されています(図“ QIAsymphony DSP Virus/Pathogen操作”)。ワークテーブルのセットアップが簡単かつ迅速に行なえ、貴重な時間を節約できます。QIAsymphony SP のボックスに最高2種類の試薬カートリッジを入れるだけで準備完了です。同じキットの試薬カートリッジではなく、別々のキットの試薬カートリッジをセットすれば、一回のランで96サンプルから2種類の精製工程が可能です。選択したサンプル数だけの試薬量が使用されるため、コストを完璧にコントロールできます。

QIAsymphony DSP Virus/Pathogen Kitとともに使用するプロトコール
プロトコール キット サンプル サンプル容量
Cellfree 200 QIAsymphony DSP Virus/Pathogen Mini Kit 血清、血漿、CSF 200 µll
Cellfree 500 QIAsymphony DSP Virus/Pathogen Midi Kit 血清、血漿、CSF 500 μl
Cellfree 1000 QIAsymphony DSP Virus/Pathogen Midi Kit 血清、血漿、CSF 1000 μl
Complex 200 QIAsymphony DSP Virus/Pathogen Mini Kit 呼吸器系検体(BAL、乾燥スワブ、輸送保存液、吸引液、痰)および泌尿生殖器検体(尿、輸送保存液) 200 μl
Complex 400 QIAsymphony DSP Virus/Pathogen Midi Kit 呼吸器系検体(BAL、乾燥スワブ、輸送保存液、吸引液、痰)および泌尿生殖器検体(尿、輸送保存液) 400 μl
Complex 800 QIAsymphony DSP Virus/Pathogen Midi Kit 呼吸器系検体(BAL、乾燥スワブ、輸送保存液、吸引液、痰)および泌尿生殖器検体(尿、輸送保存液) 800 μl
図参照

アプリケーション

QIAsymphony DSP Virus/Pathogen Kit は様々なDNA およびRNA ウイルス、また病原性細菌を高感度で検出します。直線性のある収量が得られ、ウイルスあるいはバクテリアは力価にかかわらず正確な定量解析が可能です。高品質な核酸は、リアルタイム定量 PCRあるいはRT-PCRを用いた高感度検出を含むすべてのダウンストリームアプリケーションで即使用可能です。

裏付けデータと数値

リソース

MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
パフォーマンスデータ (2)
QIAsymphony DSP Virus/Pathogen Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Application/Protocol Documents for User-Validated Use (6)
Application/Protocol Documents for IVD Use (9)
Complex400_OBL_V4_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Cellfree500_V5_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Complex200_V6_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Complex800_V6_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Cellfree1000_V7_DSP protocol


QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Cellfree200_V7_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Complex400_V4_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Complex800_OBL_V4_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Complex200_OBL_V4_DSP protocol

QIAsymphony DSP Virus/Pathogen Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
実験器具関連資料 (1)
QIAsymphony DSP Virus/Pathogen Kit Labware List_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
キットハンドブック (1)
QIAsymphony DSP Virus/Pathogen Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
Tube carriers for up to 24 tubes (primary or secondary tubes or tubes containing internal controls) can be used. A list of compatible labware formats for sample input can be found at www.qiagen.com/goto/dsphandbooks. Slots 1–4 are used for samples to be processed. The internal control-carrier RNA-Buffer AVE mixture is loaded into Slot 5.
FAQ ID -2936
Why do I have to use an ACS (Assay Control Set)?
The ACS specifies the combination of a protocol and a predefined carrier RNA–internal control mixture. The user can define an internal control mixture using the QIAsymphony Management Console. A predefined internal control-carrier RNA-Buffer AVE mixture can be automatically identified by the instrument using a bar code label or it can be chosen manually.
FAQ ID -2933
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
We recommend using racked Elution Microtubes CL (cat. no. 19588) for elution. Alternatively, 2 ml Sarstedt tubes (with screw caps, Sarstedt cat. no. 72.693) can be used. A list of compatible labware formats for eluates can be found at www.qiagen.com/goto/dsphandbooks.
FAQ ID -2937
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?

The QIAsymphony DSP Virus/Pathogen Mini Kit is used with the Cellfree200, Complex200 and Complex200_OBL protocols.

The QIAsymphony DSP Virus/Pathogen Midi Kit is used with the Cellfree500, Cellfree1000, Complex400, Complex400_OBL, Complex800, and Complex800_OBL protocols.

All complex protocols require Buffer ATL, which is not included in the QIAsymphony DSP Virus/Pathogen Kits. Buffer ATL can be ordered separately (Buffer ATL (4 x 50 ml), cat. no. 939016).

FAQ ID -2930
Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
Yes. The QIAsymphony DSP Virus/Pathogen Kits are designated “For In Vitro Diagnostic Use”. It is the user’s responsibility to validate system performance for any procedures used in their laboratory that are not covered by the QIAGEN performance evaluation studies. The system performance has been established in performance evaluation studies purifying viral DNA and RNA from human serum, plasma, or cerebrospinal fluid (CSF), and purifying viral DNA and RNA, as well as bacterial DNA, from respiratory and urogenital samples. To minimize the risk of a negative impact on the diagnostic results, adequate controls for downstream applications should be used. For further validation, the guidelines of the International Conference on Harmonisation of Technical Requirements (ICH) in ICH Q2 R(1) Validation of Analytical Procedures: Text and Methodology are recommended. Any diagnostic results that are generated must be interpreted in conjunction with other clinical or laboratory findings.
FAQ ID -2939
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.
FAQ ID -2928
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
For all cellfree and complex protocols, an eluate volume of 60 µl, 85 µl, or 110 µl can be selected. These are the minimum accessible volumes of eluate in the final elution tube. The initial elution volumes, which have to be used for the internal control, as well as titer calculations, are 90 µl, 115 µl, and 140 µl.
FAQ ID -2938
Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
Pretreatment can improve the nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Performance may be improved for Gram positive bacteria by pretreatment with lysozyme. Pretreatment protocols are provided in the QIAsymphony DSP Virus/Pathogen Handbook.
FAQ ID -2931
How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
The QIAsymphony DSP Virus/Pathogen Mini kit can be used to process 192 samples (96 per reagent cartridge). The Midi kit can be used to process 96 samples (48 per reagent cartridge) using the Cellfree1000, Complex800, and the Complex800_OBL protocols. The Midi Kit can also be used to process 144 samples (72 per reagent cartridge) using the Cellfree500, Complex400, and the Complex400_OBL protocols.
FAQ ID -2935
What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols?

The sample volume depends on the QIAsymphony DSP Virus/Pathogen protocols:

  • Cellfree200 — 200 µl
  • Cellfree500 — 500 µl
  • Cellfree1000 — 1000 µl
  • Complex200 and Complex200_OBL — 200 µl
  • Complex400 and Complex400_OBL — 400 µl
  • Complex800 and Complex800_OBL — 800 µl
FAQ ID -2929
How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
Sample material can be stored at 2–8°C for up to 6 h. For longer storage, samples should be kept at –20°C or –70°C.
FAQ ID -2934
Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
No. The use of internal controls is not mandatory, but is strongly recommended. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).
FAQ ID -2932
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