RT² lncRNA qPCR Assay

RT² lncRNA qPCR Assays are designed for SYBR^® Green based, real-time PCR detection of long, non-coding RNAs. Assays are available for almost all human and mouse long non-coding genes annotated by NCBI and GENCODE.

RT² lncRNA PCR Assays are designed using a computer algorithm which has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size without primer-dimers or off-target amplification in 33 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is 100 +/– 10%. As a result, the algorithm designs highly effective primer sequences for SYBR Green based real-time PCR detection. RT² lncRNA qPCR Assays are available for almost all human and mouse long non-coding genes annotated by the NCBI and GENCODE (see QIAGEN website for the version coverage).

For optimal performance, RT² lncRNA qPCR Assays should be used together with the RT² First Strand Kit for cDNA synthesis and RT² SYBR Green Mastermixes for qPCR. These reagents have been formulated and pretested together with RT² lncRNA qPCR Assays. The RT² First Strand Kit includes a proprietary genomic DNA elimination step to remove any residual contamination in RNA samples before reverse transcription, thereby eliminating false positive signals. In addition, an artificial RNA control template is also included which can be used to follow the reverse transcription efficiency across samples and monitor against RNase contamination. Each of the real-time instrument-specific RT² SYBR Green Mastermixes contains SYBR Green and an appropriate reference dye to match the instrumentation available in your laboratory. RT² SYBR Green Mastermixes are available for all real-time PCR instruments from QIAGEN, Applied Biosystems, Bio-Rad, Stratagene, Eppendorf, Roche, and other major suppliers.

RT² lncRNA PCR Assays are highly suited for detection of human and mouse long non-coding RNAs.