ipsogen NPM1 mut A, B&D MutaQuant Kits

For quantification of type A, B, and D mutations in NPM1 gene transcripts using real-time PCR

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ipsogen NPM1 mut B&D MutaQuant Kit (24)

Cat. No. / ID: 677613

For 24 reactions: ABL Control Gene Standards, NPM1 MutB Gene Standards, NPM1 MutD Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutB Mutated Gene, Primer and Probe Mix NPM1 mutD Mutated Gene

ipsogen NPM1 mut A MutaQuant Kit (24)

Cat. No. / ID: 677513

For 24 reactions: ABL Control Gene Standards, NPM1 MutA Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutA Mutated Gene

Features

Sensitive quantitative assay
Specific quantification of type A, B, and D mutations
Accurate quantification relative to ABL control gene expression
Optimal quality control and ISO 13485 certification
Easy workflow with ready-to-use solutions

Product Details

ipsogen NPM1 mut A, B&D MutaQuant Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. ipsogen NPM1 mut A, B&D MutaQuant Kits provide reagents optimized for reliable and sensitive detection and quantification of specific mutations (type A, B, and D) in NPM1 gene transcripts.

Performance

To ensure highest sensitivity, ipsogen NPM1 mut A, B&D MutaQuant Kits have been optimized to detect the type A, B, and D mutations in NPM1 gene transcripts over a wide dynamic range (see figure "Sensitive detection of ABL and NPM1 mut A, B, and D standards").

Principle

ipsogen NPM1 mut A, B&D MutaQuant Kits are ready-to-use kits for the detection and quantification of type A, B, and D mutations in human RNA already identified as carrying the respective mutation in the NPM1 gene (see figure " NPM1 alleles"). Results are quantified relative to expression of the ABL control gene (see figure " ABL control gene transcript"). The kits provide high levels of sensitivity and reproducibility. Each ipsogen NPM1 mut A, B&D MutaQuant Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for each of the NPM1 gene mutations.

See figures

NPM1 alleles.

ABL control gene transcript.

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of DNA by real-time PCR. Using ipsogen NPM1 mut A, B&D MutaQuant Kits provides reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

ipsogen NPM1 mut A, B&D MutaQuant Kits enable sensitive and reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts for research use only.

Supporting data and figures

Sensitive detection of NPM1 mut A and ABL standards.

Detection of [A] NPM1 mut A standards (M1 to M5) with 10¹, 10², 10³, 10⁵, and 10⁶ copies/5 μl and [B] ABL standards (C1, C2, C3) with 10³, 10⁴, and 10⁵ copies/5 μl.