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miRCURY LNA miRNA QC PCR Panel

qPCRプロファイリングにおけるmiRNAサンプルの迅速かつ簡便なRNA品質管理

S_1084_5_GEN_V2
GeneGlobeで探索・設定する
適切なターゲット特異的アッセイおよびパネルを探すか、またはターゲットをカスタムデザインし、お客様のご興味のある生物学的ターゲット評価にお使いください。

miRCURY LNA miRNA QC PCR Panel

Cat. No. / ID:   339331

miRCURY LNA miRNA PCR Panel of quality control assays, available in 96-well or 384-well format; for SYBR® Green-based detection
GeneGlobeで探索・設定する 価格を確認するには
GeneGlobeで探索・設定する
miRCURY LNA miRNA QC PCR Panelは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。
GeneGlobeで探索・設定する
適切なターゲット特異的アッセイおよびパネルを探すか、またはターゲットをカスタムデザインし、お客様のご興味のある生物学的ターゲット評価にお使いください。

特徴

  1. サンプル調製、cDNA合成およびqPCRを評価するためのオールインワンソリューション
  2. 厳選された生物学的マーカーと膨大なデータに基づくスパイクイン
  3. 血清/血漿サンプルの溶血の程度を示すユニークな溶血インジケーター
  4. ヒト、マウス、ラット、サル、およびイヌに対する包括的にカバー


製品詳細

miRCURY LNA miRNA QC PCR Panelは、RNAサンプルの迅速かつ簡便な品質管理が可能です。そのため、qPCRプロファイリング研究に最適なサンプルのみを確実に使用することができます。 分離したRNAの品質をチェックして規定値外のサンプルを探したり、ヌクレアーゼとPCRインヒビターの存在を確認し、溶血の兆候を探すために、このPCRパネル(96または384ウェルフォーマット)をご利用ください。 これにより無駄な時間を削減し、最適なサンプルで実験を始められます。

研究プロジェクトでの見積もりが必要な場合や、専門チームとプロジェクトについて相談したい場合は、こちらよりお気軽にご相談ください。

パフォーマンス

他に類を見ない製品

miRCURY LNA miRNA QC PCR Panelは、サンプル品質とPCR効率を確認するためのユニークで包括的な手法が盛り込まれています。 このパネルは、培養細胞、組織サンプル、ヒト、マウス、ラット、サルおよびイヌの体液など、多くのサンプルタイプと互換性があります(miRCURY LNA miRNA QC PCR Panels can be used with a wide range of sample types 参照)。

 

このパネルを使用すれば、溶血の影響を受けた血清/血漿サンプル(Determine RNA yields using the RNA Spike-in Kit 参照)を含む不適格なサンプル(Assessing if samples are affected by hemolysis 参照)を見出すことができます。 これにより、研究室の時間とリソースが節約されます。

原理

The importance of a good start

miRNA qPCR is the most commonly used technique for miRNA expression profiling. There are many advantages of using PCR: it is fast, sensitive and accurate. However, successful qPCR experiments depend on high-quality input RNA. Degraded RNA decreases the efficiency of the technique, and inhibitors can adversely affect the cDNA synthesis and amplification steps. For some samples, such as biofluids, determining the quality of the purified RNA can be difficult.
The miRCURY LNA miRNA QC PCR Panel offers a unique and comprehensive way to check your sample quality and PCR efficiency. The panel contains LNA-enhanced PCR primer sets that target miRNAs and spike-ins that can be specifically used for RNA quality control. These primer sets have been selected based on our extensive experience in miRNA qPCR and analysis of millions of datapoints.
When used in conjunction with the RNA Spike-in Kit, the panel can help you find differences in RNA yield and identify outlier samples (see figure  Quickly identify outlier RNA samples). In addition, RNA spike-ins can be used to check the cDNA synthesis and PCR amplification efficiencies.
The miRCURY LNA miRNA QC PCR Panel contains assays for miRNAs that are expressed in a wide range of sample types and is therefore suitable for evaluating the samples in a biological context. Two of the miRNAs in the panel can be used to evaluate the level of hemolysis in samples such as serum and plasma.

Product coverage

The miRCURY LNA miRNA QC PCR Panel consists of 12 predefined assays for use in human, mouse, rat, monkey and dog samples (see figure  Panel layout and table miRCURY LNA miRNA QC PCR Panel coverage). The panel can be used for assessing RNA from a wide variety of samples, including tissue, cell culture and biofluids.
The panel is available in two formats:

  • 96-well, ready-to-use: two 96-well PCR plates containing 16 miRNA QC panels; compatible with most real-time PCR instruments.
  • 384-well, ready-to-use: one 384-well PCR plate containing 32 miRNA QC panels; compatible with most real-time PCR instruments.

The plates are produced in a ready-to-use format, which means that the primer sets are dispensed and lyophilized in the well in an amount sufficient for one 10 µl reaction per well.

 

miRCURY LNA miRNA QC PCR Panel coverage
Content Assay Function
miRNA assay miR-103 Biological marker found in most tissues
miRNA assay miR-103 Biological marker found in most tissues
miRNA assay miR-191 Biological marker found in most tissues
miRNA assay miR-451 Marker of hemolysis in serum/plasma
miRNA assay miR-23a Internal control for hemolysis in serum/plasma
RT assay UniSp6 Monitor cDNA synthesis
Spike-in assay UniSp2 Control for the quality of the RNA isolation
Spike-in assay UniSp4 Control for the quality of the RNA isolation
Spike-in assay UniSp5 Control for the quality of the RNA isolation
RT assay cel-miR-39-3p Monitor cDNA synthesis
miRNA assay miR-30c Biological marker found in kidney
miRNA assay miR-124 Biological marker found in cerebrospinal fluid
Interplate calibrator/PCR control UniSp3 PCR efficiency control
図参照

操作手順

Analysis and interpretation of data
An important factor when using the miRCURY LNA miRNA QC PCR Panel and the RNA Spike-In Kit is knowing how to interpret the experimental data. We have made this easy by devoting an entire section in the kit handbook to explaining how to analyze and interpret the data in an easy-to-understand and clear-cut way. Refer to the miRCURY LNA miRNA QC PCR Panel Handbook for details.

アプリケーション

Quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR experiments

裏付けデータと数値

リソース

MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
クイックスタートプロトコール (2)
サイエンティフィック・ポスター (1)
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キットハンドブック (1)
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