Features
- Rapid isolation of high-quality, ready-to-use DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
Product Details
The QIAamp DNA Blood BioRobot 9604 Kit provides automated DNA purification on the BioRobot 9604 workstation using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot 9604 workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
Performance
QIAamp 96-well plates provide well-to-well uniformity in DNA recovery and purity (see figures " Reproducible DNA yield" and " Reproducible DNA purity"), and PCR experiments show no cross-contamination between samples in adjacent wells (see figure " Cross-contamination-free purification for reliable PCR"). As many as 96 samples of human whole blood or other body fluids can be processed yielding purified DNA in under 2 hours with minimal hands-on time. QIAamp sample preparation technology is fully licensed.
The QIAamp 96 DNA Blood procedure on the BioRobot 9604 increases productivity and reliability. DNA purified using the QIAamp 96 DNA Blood procedure on the BioRobot 9604 is free of contaminants and enzyme inhibitors, and is ready for use in amplification, Southern blotting, or other enzymatic assays for
See figures
Principle
No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from blood and other body fluids ready to use in PCR and blotting procedures.
Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.
Procedure
The QIAamp DNA Blood BioRobot 9604 Kit simplifies isolation of DNA from blood and other body fluids with a fast automated 96-well–plate procedure. Using the BioRobot 9604 Workstation, the QIAamp DNA Blood BioRobot 9604 Kit processes up to 200 µl sample size, with preparation time of 96 samples in less than 2 hours (see " Protocol"). The automated procedure, including bar code reading and process documentation, needs about 5 minutes hands-on time, with a turnaround time between consecutive runs of about 10 minutes. The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.
See figures
Applications
The QIAamp DNA Blood BioRobot 9604 Kit provides automated high-throughput DNA purification on the BioRobot 9604 using proven QIAamp technology. The kit processes 96-well plates for up to 96 preps. Sample types include:
- Fresh or frozen whole blood
- Serum
- Bone marrow
- Body fluids
- Cell suspensions
Supporting data and figures
QIAamp 96 DNA Blood procedure.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, blotting |
| For automated processing | BioRobot 9604 |
| Sample amount | 200 µl |
| Main sample type | Whole blood |
| Elution volume | 200 µl |
| Processing | Automated |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Format | 96-well plate |
| Time per run or per prep | <2 hours |
| Technology | Silica technology |
| Yield | 3–6 µg |
Resources
MSDS (1)
Safety Data Sheets (1)
Publications
Functional polymorphisms of the human multidrug-resistance gene: multiple sequence variations and correlation of one allele with P-glycoprotein expression and activity in vivo.
Proc Natl Acad Sci U S A; 2000; 97 (7):3473-8 2000 Mar 28 PMID:10716719
FAQ
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
How do I safely inactivate biohazardous flow-through material?