Products
Rotor-Gene Q 5plex Priority Package
Cat. No. / ID: 9001865
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Rotor-Gene Q 5plex Priority Package Plus
Cat. No. / ID: 9001866
Real-time PCR cycler with 5 channels (green, yellow, orange, red, crimson), laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Rotor-Gene Q 5plex HRM Platform
Cat. No. / ID: 9001580
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes 1-year warranty on parts and labor, installation and training not included
Rotor-Gene Q 2plex HRM Priority Package
Cat. No. / ID: 9001863
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Rotor-Gene Q 5plex HRM Priority Package
Cat. No. / ID: 9001867
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Rotor-Gene Q 2plex HRM Priority Package Plus
Cat. No. / ID: 9001864
Real-time PCR cycler and High Resolution Melt analyzer with 2 channels (green, yellow) plus HRM channel, laptop computer, software, accessories: includes includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Rotor-Gene Q 5plex HRM Priority Package Plus
Cat. No. / ID: 9001868
Real-time PCR cycler and High Resolution Melt analyzer with 5 channels (green, yellow, orange, red, crimson) plus HRM channel, laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Rotor-Gene Q 6plex Priority Package
Cat. No. / ID: 9001869
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Rotor-Gene Q 6plex Priority Package Plus
Cat. No. / ID: 9001870
Real-time PCR instrument with 6 channels (blue, green, yellow, orange, red, crimson), including laptop computer, software, accessories: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Features
- Outstanding thermal and optical performance due to rotary format
- An unmatched optical range spanning UV to infrared wavelengths
- State-of-the art analyses supported by user-friendly software
- Low maintenance and maximum convenience due to robust design
- High performance in multiple applications with QIAGEN assays
Product Details
QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications. Q-Rex Software is operating and analysis software for life science qPCR applications. A choice of plug-ins lets you add new functionality without affecting established workflows.
Explore the virtual demo to learn more about the Rotor-Gene Q.
Performance
The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM. Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure " Identification of a class IV SNP" for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure " Highly sensitive detection of methylated DNA" for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching. The Type-it HRM PCR Kit reliably and accurately detects gene mutations and SNPs. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA.
See figures
Principle
Unique rotary design for outstanding performance
The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure " Cross-section of the Rotor-Gene Q"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure " Precise real-time PCR analysis"). It also eliminates sample-to-sample variations and edge effects. These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways.
The rotary design delivers:
- Tube-to-tube variation ±0.02°C
- Uniform detection eliminating the need for ROX reference dye
- Fast ramping and negligible equilibration times for short run-times
- Complete confidence in your results
Unrivaled optical range enables multiple applications
Whether your assay is based on intercalating dyes such as SYBR® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future.
| Channel | Excitation (nm) | Detection (nm) | Examples of fluorophores detected |
|---|---|---|---|
| Blue | 365 ± 20 | 460 ± 20 | Marina Blue, Edans, Bothell Blue, Alexa Fluor 350, AMCA-X |
| Green | 470 ± 10 | 510 ± 5 | FAM, SYBR® Green I, Fluorescein, EvaGreen, Alexa Fluor 488 |
| Yellow | 530 ± 5 | 557 ± 5 | JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow |
| Orange | 585 ± 5 | 610 ± 5 | ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568 |
| Red | 625 ± 5 | 660 ± 10 | Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 633 |
| Crimson | 680 ± 5 | 712 high pass | Quasar 705, LightCycler Red705, Alexa Fluor 680 |
| HRM | 460 ± 20 | 510 ± 5 | SYBR® Green I, SYTO9, LC Green, LC Green Plus+, EvaGreen |
Expand your research with HRM
High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. PCR products can be discriminated according to sequence, length, GC content, or strand complementarity, down to single base-pair changes. Previously unknown and even complex sequence variations can be readily detected and characterized in a robust and straightforward way. The rotary design of the Rotor-Gene Q and its outstanding thermal and optical performance are highly suited to HRM.
The HRM option for the Rotor-Gene Q includes:
- A specially tuned high-intensity optical HRM channel
- Thermal resolution of 0.02°C
- High data-acquisition rates
- Comprehensive HRM software
Minimum maintenance; maximum convenience
The Rotor-Gene Q is engineered to reduce the need for maintenance and to maximize ease of use. This saves time and costs, and allows you to focus on your research; not on keeping the cycler up and running.
Convenient features of the Rotor-Gene Q include:
- Lifetime guarantee on highly stable LEDs, no expensive lamps or lasers to change, no gradual performance loss of light source
- No optical calibration needed at installation or when the instrument is moved
- No sample block to clean
- No condensation or bubbles in reactions due to rotation
- Small, light, and robust; simply place the instrument wherever you like
See figures
Procedure
Flexible formats match your workflows
The Rotor-Gene Q supports multiple PCR tube formats to suit a range of needs (see figure " Flexible formats"). Changing the format, by simply switching the snap-fit metal rotor that holds the tubes, takes just seconds. As well as tubes, Rotor-Discs are available, which offer accelerated setup and higher throughput. Rotor-Discs are circular plates of vertically oriented reaction wells. The Rotor-Disc 100 is the equivalent of a 96-well plate with an additional 4 reference wells. These extra wells can be conveniently used for more reactions or additional controls. Alternatively, the Rotor-Disc 72 has 72 wells. Rotor-Discs can be quickly and easily sealed with plastic film using a Rotor-Disc Heat Sealer. For all you need to run reactions using Rotor-Discs, choose the Rotor-Disc 100 Starter Kit or the Rotor-Disc 72 Starter Kit.
You can perform manual reaction setup, or take advantage of QIAGEN's automated solutions for reaction setup. The QIAgility is cost-effective and delivers rapid, high-precision PCR setup, while the QIAsymphony AS is ideal for laboratories performing routine PCR tests on a day-to-day basis. Both instruments perform automated reaction setup in Rotor-Gene formats, allow direct transfer of sample lists, and are supplied with verified protocols for real-time PCR master mixes.
Easy routine verification
Laboratories may often want to verify thermal accuracy. For most cyclers, this requires interaction with a service engineer. With the Rotor-Gene Q, this is not necessary. Instead, the easy-to-use, cost-effective Rotor-Disc OTV (Optical Temperature Verification) Kit automates accuracy testing. The full procedure takes only a couple of minutes.
See figures
Applications
A range of QIAGEN kits for the Rotor-Gene Q enables reliable quantification in all your real-time PCR applications without the need for optimization of reaction and cycling conditions. Kits for real-time PCR and HRM applications are available for:
- Gene expression analysis
- Pathogen detection
- DNA methylation analysis
- Genotyping and gene scanning
- miRNA research
Software
Q-Rex Software
Q-Rex Software is operating and analysis operating and analysis software that drives Rotor-Gene Q hardware and facilitates experiment setup, data acquisition and basic and advanced analyses. The configurable nature of Q-Rex allows you to easily create the exact software configuration to meet your specific analysis needs without installing functions you will never use.
- Fast and easy access to templates and recent experiments
- Drag-and-drop creation of new experiments
- Plug-ins to expand capacity for specific applications
- Audit trail for 21CFR part 11 compliance
The software is suitable for use by the most novice researchers, while maintaining the highly complex data analysis functions required by advanced researchers.
Rotor-Gene Q Software
The comprehensive Rotor-Gene Q software package supports all current state-of-the art real-time analysis procedures from basic to advanced algorithms. This provides complete freedom to analyze your valuable experimental data and increases the reliability of your results. Data security is assured and all process steps are trackable from starting the run to exporting the results.
Superior Rotor-Gene ScreenClust HRM Software available for genotyping and mutation detection using HRM analysis
Rotor-Gene ScreenClust HRM Software is an extension to the Rotor-Gene operating software. This software is the most powerful tool currently available for analysis of HRM data from the Rotor-Gene Q or Rotor-Gene 6000 cycler. By grouping samples into clusters, Rotor-Gene ScreenClust HRM Software opens a new dimension in HRM analysis for applications such as genotyping and mutation screening.
REST software 2009
REST software 2009 is a standalone software tool for analysis of gene expression data from quantitative real-time PCR experiments. REST software 2009 is available for download under the "Resources" tab, and provides valuable analysis, including:
- Estimation of up- and down-regulation for gene expression studies
- Randomization and bootstrapping techniques
- Graphical data output via whisker-box plots
Traditional relative quantification enables estimation of gene expression. However, this method does not provide statistical information that is suitable for comparing expression in groups of treated and untreated samples in a robust manner. The integrated randomization and bootstrapping methods used in REST software 2009 test the statistical significance of calculated expression ratios and can be used even when the data includes outliers.
REST software 2009 applies a mathematic model that takes into account the different PCR efficiencies of the gene of interest and reference genes. Compared to using a single reference gene, using multiple reference genes for normalization can improve the reliability of results.
Rotor-Gene Assay Manager
Rotor-Gene AssayManager is a software for routine testing. The core application is complemented by a selection of plug-ins that contain assay-type-specific analysis and visualization of the results for fully automated real-time PCR analysis. Further information is available from the following links:
Services
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Learn more about trade-in/trade-up opportunities.
Supporting data and figures
Identification of a class IV SNP.
A human A/T SNP in the AHRR7 gene was analyzed using genomic DNA from wild-type (blue), homozygous mutant (green), and heterozygous (red) samples. Experiments were performed using the Type-it HRM PCR Kit and a Rotor-Gene Q cycler with an HRM channel. Data analysis was performed with the unsupervised mode of Rotor-Gene ScreenClust HRM Software. A/T polymorphisms (class IV SNPs) are most difficult to discriminate due to minute differences between homozygote alleles (in this example, less than 0.1°C). [A] HRM raw data, [B] cluster plot. All pseudo-unknowns were correctly clustered according to genotype.
Specifications
| Features | Specifications |
|---|---|
| Dimensions | |
| Operating temperature | |
| Pollution level | |
| Humidity | |
| Features | |
| Protocols/main application on this instrument | |
| Software | |
| Overvoltage category | |
| Heat dissipation/thermal load | |
| Optical System | |
| Power | |
| Kits designed for this instrument | |
| Place of operation | |
| Samples per run (throughput) | |
| Storage conditions | |
| Technology | |
| Transportation conditions | |
| Warranty | |
| Thermal performance | |
| Typical run time | |
| Weight | |
| Altitude |
Service Plans
RGQ, Core Agreement
Cat. No. / ID: 9245253
One-time Rotor-Gene Q instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
Resources
Quick-Start Protocols (1)
Supplementary Protocols (5)
Scientific Posters (4)
Instrument Technical Documents (3)
Analysis Software (12)
Software User Guides (6)
Brochures & Guides (7)
Instrument User Manuals (9)
Technical Information (3)
Operating Software (5)
Kit Handbooks (3)
Validation Reports (2)
Application Notes (1)
Application/Protocol Documents (2)
FAQ
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
How is the hypothesis test performed in the REST software?
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
Does the Rotor-Gene Q software have the capacity to export data?
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
What is a QuantiTect Primer Assay?
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
What types of reaction vessels are required for use in the Rotor-Gene Q?
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
How long does an Investigator Quantiplex run take?
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
For which real-time cyclers has the Investigator Quantiplex Kit been validated?
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
What real-time cycler should I use for my qPCR experiments?
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
Can Vapor-Lock be used with samples processed on the QIAgility?
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
How fast does the rotor spin during a run on the Rotor-Gene Q?
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
Can the Rotor-gene software run on a computer with Windows 7 operating system?
What should I do if the Rotor-Disc OTV run does not pass?
Why is no fluorescence signal detected in my run?
What kind of file is required for hardware-related trouble-shooting?
Is regular calibration needed with the Rotor-gene instrument?
Can I import a standard curve from a previous PCR run on the Rotor-Gene Q?
Which-reporter-dyes-can-be-combined-for-use-in-multiplex-PCR-on-the-Rotor-Gene-Q-Cycler?
Must I fill blank positions with empty tubes when running sample numbers lower than the rotor capacity of the Rotor Gene Q?
What reaction volume is suitable for use in the Rotor-Gene Q?