artus WNV LC RT-PCR Kit CE

• Compliance with EU IVD Directive 98/79/EC
• 신뢰할수 있는 결과 — DNA 분리 과정과 PCR 과정이 잘 수행되었는지 증명하기 위한 internal control 이용
• 고감도 검출 — 2.4 copies/µl
• 제공되는 4개의 standard를 이용하여 WNV RNA를 정확하게 정량할 수 있음

To ensure highest sensitivity, the artus WNV LC RT-PCR Kit has been optimized to detect low numbers of West Nile virus RNA. The analytical sensitivity of the artus WNV LC RT-PCR Kit is 2.4 copies/µl in the PCR on the LightCycler 1.1/1.2/1.5 Instruments.

The artus WNV LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the West Nile virus genome using real-time PCR. The kit provides high levels of specificity, sensitivity, and reproducibility.

Each artus WNV LC RT-PCR Kit provides 4 quantitation standards (see figure " Reliable quantitation of WNV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical WNV RT-PCR is not reduced.

The artus WNV LC RT-PCR Kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.