Methylation 분석을 위한 control 실험에 사용할 수 있습니다
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
EpiTect PCR Control DNA Set (100)
Cat. No. / ID: 59695
Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs
로그인 계정 가격 보기.
EpiTect Control DNA and Control DNA Set은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품들은 질병의 진단, 예방, 또는 치료 목적으로 사용할 수 없습니다.
✓ 연중무휴 하루 24시간 자동 온라인 주문 처리
✓ 풍부한 지식과 전문성을 갖춘 제품 및 기술 지원
✓ 신속하고 안정적인 (재)주문
특징
- 편리함, 바로 사용가능, quality controlled DNA
- Pre-converted DNA로 신뢰할 수있는 methylation control 실험 보장
- 모든 DNA methylation 분석에 적합
제품 세부 정보
EpiTect Control DNAs are ready-to-use, completely methylated or completely unmethylated bisulfite converted DNAs, and untreated, unmethylated genomic DNA, for standardized and reliable control reactions for methylation analysis. The methylated or unmethylated and bisulfite converted DNAs are stored in EB Buffer (10 mM Tris·Cl) in a ready-to-use 10 ng/μl solution. The unmethylated human control DNA, which is not converted, is stored in EB Buffer (10 mM Tris·Cl) in a ready-to-use 50 ng/μl solution.
성능
EpiTect Control DNAs are highly suited for use as quantification standards for probe-based real-time PCR methylation analysis (see figure " Real-time PCR standards") and high-resolution melting (HRM) methylation analysis (see figure " HRM quantification standards").
그림 참조
원리
The EpiTect Control DNA Set contains all control DNAs needed for standardized and reliable methylation control reactions in a ready-to-use kit format . In methylation-specific PCR, for example, control reactions must be carried out to ensure that PCR probes and primers bind specifically to methylated or unmethylated DNA (see figure "EpiTect Control DNA in MSP"). These reactions require various concentrations of completely bisulfite converted control DNA that is fully methylated or fully unmethylated (see figures " EpiTect Control DNA Pyrogram" and " EpiTYPER MALDI-TOF analysis of WBA DNA"). Additionally, mixtures of these control DNAs can serve as quantification standards when determining degree of methylation in HRM and probe-based methylation experiments.
그림 참조
절차
Standardized workflows in epigenetics
Accessing epigenetic information is of prime importance for many areas of biological and medical research — particularly oncology, but also stem cell research and developmental biology. However, the analysis of changes in DNA methylation is challenging, due to the lack of standardized methods for providing reproducible data particularly from limited sample material. With its newly introduced EpiTect solutions, QIAGEN makes available standardized, pre-analytical and analytical solutions from DNA sample collection, stabilization and purification, to bisulfite conversion and real-time or end-point PCR methylation analysis or sequencing (see figure " Standardized workflows in epigenetics").
그림 참조
응용 분야
EpiTect Control DNAs are suitable for use in control reactions for all types of methylation analyses, including:
- Evaluating primer specificity for MSP
- Quantification standard for HRM and MethyLight PCR
- Evaluating primer and probe specificity for MethyLight PCR
- Checking efficiency of bisulfite conversion reactions
지원되는 데이터 및 수치
Real-time PCR standards.
EpiTect Methylated Control DNA and EpiTect Unmethylated Control DNA (both pre-bisulfite converted) were mixed to give 100%, 90%, 50%, 10%, and 0% methylated DNA. EpiTect MethyLight Assays for the human PITX2 gene were run in triplicate, using 10 ng of each DNA sample. The results show that EpiTect Control DNA can be used as a methylation standard for the quantification of unknown DNA samples.
Specifications
| Features | Specifications |
|---|---|
| Applications | End point Methylation Specific PCR (MSP), real-time methylation specific PCR |
| Number of reactions | for 1000 control PCRs |
| Concentration | 10 ng/µl (1 µg in total) excepted the unmethylated control DNA: 50 ng/µl (10 µg in total) |
리소스
Kit Handbooks (4)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
3341 - How are the EpiTect DNA controls produced and tested?