Products
The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.
Features
- Rapid analysis of up to 96 samples without manual intervention
- Safety and convenience with ready-to-use gel cartridges
- Accurate analysis of low concentration nucleic acids with 3–5 bp resolution
- User-friendly analysis software that supports electronic signatures and records
- Objective RNA quality measurement with new RNA Integrity Score (RIS)
Product Details
The revolutionary QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA — streamlining workflows and reducing time to result. The QIAxcel Advanced system fully automates sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. DNA fragment analysis of 12 samples can be performed in as little as 3 minutes. Ready-to-run gel cartridges allow 96 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for tedious gel preparation. User-friendly QIAxcel ScreenGel software ensures convenient analysis and documentation of data. The QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement of the analyzed samples and allows easy interpretation of sample integrity.
The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0.
Performance
Streamlined workflow
The QIAxcel Advanced system not only speeds up DNA and RNA analysis by eliminating slab-gel analysis, but also contributes to streamlining the entire sample purification and analysis workflow. Used in combination with proven QIAGEN end-point PCR kits, the QIAxcel Advanced provides an all-in-one, pretested solution for reliable analysis of PCR fragments, ensuring reproducibility and significant time and cost savings.
One instrument, many applications
The QIAxcel Advanced system is a versatile solution for electrophoresis and offers a broad range of applications. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids – including single or multiple PCR fragments, DNA digested with restriction endonucleases, total RNA, and cRNA. Unlike other commercially available devices that provide processing of only up to 12 samples at time, the QIAxcel Advanced system allows processing of all 96 samples without manual intervention.
DNA analysis
In genotyping applications, the QIAxcel Advanced system enables greater sizing accuracy and more sensitive detection than conventional agarose gel electrophoresis (see figure Accurate, high-throughput genotyping of bacteria). QIAxcel Advanced also provides a straightforward and effective means for checking the quality of genomic DNA (gDNA) samples prior to molecular screening and downstream assays (see figure Assessment of DNA degraded by sonication).
RNA analysis – objective quality values with the RNA integrity score
The new QIAGEN RNA Integrity Score (RIS) provides an objective quality measurement for eukaryotic RNA samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.
Such careful monitoring of the initial RNA sample, as well as products generated throughout the entire procedure, is crucial in microarray analysis, since RNA degradation strongly influences the predictive power obtained from the microarray data. The QIAxcel Advanced System is highly suitable for analyzing the integrity of total RNA and size distribution of fragmented or intact cRNA (see figure Streamlined RNA analysis using the QIAxcel system), and the throughput capacity makes it very convenient for laboratories employing 96-well RNA purification technologies that need a fast solution for quality control.
See figures
Principle
The QIAxcel Advanced instrument includes an array of light-emitting diodes and micro-optical collectors that latch to capillaries within QIAxcel gel cartridges. Fragments that are migrating through a gel matrix within the capillary pass excitation and detection spots and the signal is transmitted through a photomultiplier tube to the QIAxcel ScreenGel software for data interpretation (see figure Sample separation process).
The high detection sensitivity provided by the QIAxcel Advanced instrument enables robust results even with low concentrations of nucleic acid. With a resolution of 3–5 bp for fragments smaller than 0.5 kb, the QIAxcel Advanced system ensures greater accuracy than slab-gel methods, as well as greater confidence in data interpretation. Sample consumption is less than 0.1 μl per analysis, saving precious samples for further downstream analysis. Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals, such as ethidium bromide (see figure Ready-to-run gel cartridges).
A range of QIAxcel Kits is available for a variety of applications, meeting different resolution and speed requirements (see table below).
| QIAxcel Kit | Analyte | Size range | 100 bp – 500 bp* | 500 bp – 1 kb* | 1 kb – 5 kb* | 5 kb – 10 kb* | Run time/ 12 samples† |
|---|---|---|---|---|---|---|---|
| QIAxcel DNA High Resolution Kit | DNA | 15 bp–10 kb | 3–5 bp | 50 bp | 200–500 bp | 1–1.5 kb | 7–20 min |
| QIAxcel DNA Screening Kit | DNA | 15 bp–5 kb | 20–50 bp | 50–100 bp | 500 bp | – | 5 min |
| QIAxcel DNA Fast Analysis Kit | DNA | 15 bp–3 kb | 50–100 bp | 100–250 bp | 250 bp – 1 kb‡ | – | 3–5 min |
| QIAxcel RNA QC Kit v2.0 | RNA | 15 b–6 kb | – | – | – | – | 10 min |
See figures
Procedure
Just a few, simple steps are required to operate the QIAxcel Advanced system for analysis of DNA and RNA samples: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used — and go! Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen (see figure Reduced run times).
See figures
Applications
The QIAxcel Advanced System offers a broad range of optimized applications for DNA and RNA analysis and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids — including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.
The QIAxcel Advanced System has been used for applications such as:
- Bacterial genotyping
- PCR fragment analysis
- Pre-sequencing testing
- Mutation detection
- Detection of food allergens
- Quality control of genomic DNA
- Total RNA and cRNA QC for RT-PCR and microarray analysis
Software
QIAxcel ScreenGel software provides a new level of convenience
QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users (see figure Simplified and standardized data collection and analysis).
Easy setup and start
The Process Wizard simplifies experiment startup (see figure Easy-to-use software features). This convenient feature enables guided setup and allows run parameters to be defined and DNA size markers to be selected. Reagent lot number information can be included. Samples can be selected with a single click of the mouse and run checks can also be easily performed.
New RNA integrity score
The updated QIAxcel ScreenGel software now features the QIAGEN RNA Integrity Score (RIS), which enables objective quality measurement of analyzed samples and allows easy interpretation of sample integrity. The RIS is a value from 1 to 10 where a value of 10 indicates completely intact RNA (see figure RIS indicates RNA integrity). The highly reproducible RIS eliminates the need for human interpretation and enables the implementation of rigorous quality control at appropriate breakpoints across workflows. This allows researchers to eliminate samples that do not meet the quality criteria required for downstream processes, reducing the risk of needing to repeat experiments and saving time and costs.
Support of 21 CFR Part 11 compliance
QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:
- Password-protected login to prevent unauthorized access and data manipulation
- Audit trail documentation for configuration files and system events
- Automatic saving and archiving of write-protected raw data
- Secure user management
Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.
See figures
Services
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Learn more about trade-in/trade-up opportunities.
Supporting data and figures
RIS indicates RNA integrity.
Total RNA was extracted from rat liver samples using the RNeasy Plus Mini Kit and subjected to decomposing conditions. Samples of increasing levels of degradation were analyzed on the QIAxcel Advanced and the Agilent 2100 Bioanalyzer platform. The results are depicted in the gel view pictures for the QIAxcel Advanced [A] and the Agilent Bioanalyzer [B]. The electropherogram of selected samples analyzed on the QIAxcel Advanced is shown in [C]. The RIS (QIAxcel Advanced) and RIN (Agilent Bioanalyzer) numbers are listed in the table.
Service Plans
QIAxcel, Full Agreement
Cat. No. / ID: 9242699
Instrument repair service for the QIAxcel at regional repair center. Shipping, labor and parts included during the Full Agreement period. Loaner instrument provided within 2–3 business days. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service during the Full Agreement period.
QIAxcel Advanced, Core Agreement
Cat. No. / ID: 9245252
One-time QIAxcel Advanced instrument repair performed at a regional repair center including shipping, labor, and spare parts for a period of one year. Instrument repair turnaround time of 7–10 business days. Includes one on-site Preventive Maintenance or Inspection Service and a 10% discount on additional repair service during the Core Agreement period.
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Operating Software (1)
Brochures & Guides (3)
Additional Resources (8)
Instrument User Manuals (2)
Instrument Technical Documents (1)
White Papers (2)
FAQ
Why does the QIAxcel System or QIAxcel Advanced need to be calibrated?
Why is no signal detected in a lane?
Why do I hear a grinding sound during transportation when first installing the QIAxcel System or QIAxcel Advanced?
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
What should I do if I hear a 'hissing" sound when the QIAxcel System or QIAxcel Advanced is purging?
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
What is the QIAxcel Advanced System?
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
Why are bands compacted together on the gel image
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
When should the N2 bottle used with the QIAxcel Advanced System be changed?
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
Why is the buffer tray not moving or making a grinding sound?
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special considerations?
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
I’m getting the low pressure error on a brand new nitrogen cylinder — why?
What are the common causes of low signal?
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?