artus WNV LC RT-PCR Kit CE

• Compliance with EU IVD Directive 98/79/EC
• High reliability using the internal control
• Highly sensitive detection of as few as 2.4 copies/µl
• Accurate quantitation using the 4 standards supplied

To ensure highest sensitivity, the artus WNV LC RT-PCR Kit has been optimized to detect low numbers of West Nile virus RNA. The analytical sensitivity of the artus WNV LC RT-PCR Kit is 2.4 copies/µl in the PCR on the LightCycler 1.1/1.2/1.5 Instruments.

The artus WNV LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the West Nile virus genome using real-time PCR. The kit provides high levels of specificity, sensitivity, and reproducibility.

Each artus WNV LC RT-PCR Kit provides 4 quantitation standards (see figure " Reliable quantitation of WNV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical WNV RT-PCR is not reduced.

The artus WNV LC RT-PCR Kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.