Hybrid Capture 2 Modular System

For measurement and analysis of Hybrid Capture 2 assays

Products

The Hybrid Capture 2 Modular System is intended to be used only in combination with QIAGEN Kits indicated for use with the Hybrid Capture 2 Modular System for applications described in the respective QIAGEN Kit handbooks.

Microplate Heater I

Cat. No. / ID: 6000-1110U

96-well microplate heater, 115 V

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Rotary Shaker I

Cat. No. / ID: 6000-2240E

Rotary Shaker, 240 V

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Microplate Heater I

Cat. No. / ID: 6000-1240U

96-well microplate heater, 240 V

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LumiCheck Plate User Package

Cat. No. / ID: 6000-5013

Plate and software for use with the DML 2000 or DML 3000

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MST Vortexer 2

Cat. No. / ID: 6000-5021

Multi-Specimen tube vortex, 120 V

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MST Vortexer 2

Cat. No. / ID: 6000-5022

Multi-Specimen tube vortex, 240 V

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Automated Plate Washer

Cat. No. / ID: 6000-00174

96-well automated plate washer, 120 V

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Automated Plate Washer

Cat. No. / ID: 6000-00175

96-well automated plate washer, 240 V

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DML 3000

Cat. No. / ID: 5000-00031

Microplate luminometer, 110 V – 240 V

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HC2, Preventive Subscription

Cat. No. / ID: 9243539A

One on-site Preventive Maintenance or Inspection Service visit for the Hybrid Capture 2 System, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

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Features

Detects RNA–DNA hybrids
Accurate and flexible
For routine detection of DNA to assist in diagnosis of infectious disease

Product Details

The Hybrid Capture 2 Modular System automates some of the manual steps in processing Hybrid Capture 2 assays. The system consists of the Rotary Shaker I, Microplate Heater I, DML instrument, Automated Plate Washer, Multi-Specimen Tube (MST) Vortexer 2, and customer-configured PC preinstalled with Hybrid Capture Software. More information about customer-configured PC options is available by calling customer service. The Hybrid Capture 2 Modular System is not available in all countries.

Performance

The Hybrid Capture 2 Modular System is for use with Hybrid Capture 2 (HC2) technology, such as the digene HC2 High-Risk HPV DNA Test, digene HC2 CT-ID DNA Test, and digene HC2 GC-ID DNA Test. The system processes specimens collected in Specimen Transport Medium (STM), which is part of the digene Cervical Sampler. Other digene tests or sample types may be available for use with the Hybrid Capture 2 Modular System, depending on country regulations.

Principle

Hybrid Capture 2 (HC2) technology detects RNA–DNA hybrids using signal-amplified, chemiluminescent technology. Hybrid Capture 2 delivers the accuracy and flexibility necessary for routine detection of DNA that can assist in the diagnosis of infectious diseases. The Hybrid Capture 2 Modular System includes an automated plate washer, a luminometer, microplate heater, rotary shaker, and vortexer to automate some of the steps of manually processing Hybrid Capture 2 assays.

Procedure

The Hybrid Capture 2 Modular System automates the following manual steps for processing HC2 assays:

Microplate mixing
Microplate incubation
Microplate washing
Signal detection and results reporting

The Hybrid Capture 2 Modular System provides solutions to manual processing of the HC2 assays. The Multi-Specimen Tube (MST) Vortexer 2 is designed specifically for vortexing Hybrid Capture Specimen Collection Tubes, using a digene Specimen Rack, Conversion Rack, or a Multi-Specimen Tube Rack (see figure "MST Vortexer 2"). The Microplate Heater I (see figure " Microplate Heater I") is for incubation of samples with a Hybridization Microplate. During Hybrid Capture, the plates can be shaken on the Rotary Shaker I (see figure " Rotary Shaker I"). After Hybrid Capture, the Automated Plate Washer (see figure "Automated Plate Washer") can be used to wash the capture plate.

Amplified chemiluminescent signal detection and result reporting are performed using the DML instrument (see figure " Luminometer") with the digene Hybrid Capture System Version 2 (DHCS v.2) Software. To ensure proper calibration, use the LumiCheck Plate with LumiCheck Sotware to monitor the DML instrument performance.

The Hybrid Capture 2 Modular System is intended to be used only in combination with QIAGEN kits indicated for use with the Hybrid Capture 2 Modular System for applications described in the kit handbooks.

See figures

Microplate Heater I.

Rotary Shaker I.

Luminometer.

Applications

A range of digene HC2 DNA Tests enable detection of pathogens using the Hybrid Capture 2 Modular System. Using kits for Hybrid Capture applications, the Hybrid Capture 2 Modular System detects:

High-risk human papillomavirus (HPV) infection
Chlamydia trachomatis (CT) infections
Neisseria gonorrhoeae (GC) infections

Software

The HC2 System Software reports qualitative assay results with a user-friendly interface. Software supports customizable report formats — sorted by plate or specimen, with summary or detailed outputs. The software comes on a digene Hybrid Capture 2 System Software CD, which includes the software, digene Hybrid Capture 2 System Assay Protocols, and luminometer plate software.

Supporting data and figures

Microplate Heater I.

The Microplate Heater I is an electronically controlled heating unit that is designed to accept 96-well microplates when used with digene HC2 DNA Tests. The dual heating elements and heat capacity of the block have an integrated heater temperature controller that simultaneously turns each of the heaters on and off to maintain the desired temperature. The heater temperature controller has an electronic display readout, for ease of use.