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Investigator STAR Lyse&Prep Kit (400)
Cat. No. / ID: 931447
For 400 preps from casework and reference samples: Buffer ATL, Buffer QSL3, Buffer QSW1, Buffer QSW2, Bead Suspension G, Buffer ATE, Proteinase K, Carrier RNA, Q-Card
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This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
The Investigator STAR Lyse&Prep Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Purification from forensic and human identity samples
- Compatible with third party open DNA extraction platforms
- Efficient yields from trace casework samples
- Designed for efficient and safe use in high-throughput laboratories
- Contains all reagents for lysis, binding, washing and elution of samples
Product Details
The Investigator STAR Lyse&Prep Kit enables automated purification of genomic DNA from forensic samples using open DNA extraction platforms from third party supplier such as Hamilton, TECAN, or others. The kit format is specially designed for high-throughput applications accelerating the laboratory workflow and producing high quantities of high-quality DNA for downstream processing. The Investigator STAR Lyse&Prep Kit meets ISO 18385 requirements.
Performance
The Investigator STAR Lyse&Prep Kit enables highly efficient purification of DNA from forensic casework samples in a high-throughput setup. The kit includes sufficient reagents for 400 samples and includes multiple bottles of buffers and enzymes measured to enable utilization of one bottle per sample prep batch of up to 96 samples. This ensures efficient use of reagents and minimal storage of opened reagents, reducing any risk of contamination. Input volumes can be adjusted to accommodate a larger lysis volume for larger samples and use of the Investigator Lyse&Spin Basket Kit.
Principle
The Investigator STAR Lyse&Prep Kit reproducibly automate purification of genomic DNA, encountered in forensic and human identity applications. The purification procedure is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis. It is designed to ensure safe and reproducible handling of precious samples, and comprises 4 steps: lyse, bind, wash and elute.
Since the type of samples that can be processed using the Investigator STAR Lyse&Prep Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions, in the presence of proteinase K and Buffer ATL, in total volumes of either 300 µl (intended for routine use) or 500 µl (facilitating the use of the Investigator Lyse&Spin Basket Kit and also processing of larger samples).
Magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles, in a format designed specifically for open DNA extraction platforms. DNA is isolated from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet. The DNA is then efficiently washed and eluted in modified TE buffer (Buffer ATE).
Applications
The high-quality DNA obtained using the Investigator STAR Lyse&Prep Kit is suitable for direct use in forensic and human identity testing for applications, such as:
- Genotyping, including fingerprinting and paternity analysis
- DNA purification from trace samples (e.g., crime-scene)
- Routine analysis of reference samples
Supporting data and figures
DNA extraction and quantification from samples with added contaminants using the Investigator STAR Lyse&Prep Kit
Humic acid (1000 ng/µl) and hematin (3500 µM) were spiked into neat saliva. Five replicates were processed. DNA yield (dark blue bars, ng/µl) and the Ct values (light blue bars) of the internal control of the Investigator Quantiplex Pro RGQ Kit are shown.
Specifications
| Features | Specifications |
|---|---|
| Applications | STR analysis, (real-time) PCR |
| Sample type | Wide range of forensic and human-identity sample materials |
| Technology | Magnetic-particle technology |
| Input volume | 300 or 500 µl lysate |
Resources
Application Notes (3)
Additional Resources (3)
Validation Reports (3)
White Papers (1)
Safety Data Sheets (1)
Quick-Start Protocols (1)
Technical Information (2)
Kit Handbooks (1)
Supplementary Protocols (1)
Certificates of Analysis (1)
FAQ
What is the composition of the binding buffer?
Can I use DNA-free water to elute my samples rather than the ATE buffer?
Why is there difference in temperature for the wash steps between the manual and automated methods?
After the air-drying steps, I can still see liquid. Should I continue to dry my samples?
Can I make the binding buffer in advance?