One-step Probe RT-qPCR Mixes

• Detects low copies of RNA targets from a broad range of samples
• Exhibits consistent amplification across a wide dynamic range
• Eliminates non-specific amplification through precisely selected anti-Taq antibody
• Has a high efficiency in multiplex reactions
• Detects molecular targets accurately as fast as 40 minutes with two or three steps cycling profiles

One-Step Probe RT-qPCR Mix is a convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan ®, Scorpions ® and molecular beacon probes.

It was created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for probe-based Real-Time PCR assays, including singleplex and multiplex gene expression studies.

Using high-affinity antibodies for hot-start polymerase ensures higher specificity by reducing the formation of primer-dimer structures. It allows for a more comprehensive dynamic range by removing competition for reaction reagents. It also leads to higher sensitivity and reproducibility.

Precisely optimized buffer components ensure optimal reverse transcriptase and hot-start polymerase activity conditions. Additionally, RNase Inhibitor protects RNA from unspecific RNases.

The One-Step Probe RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly used Real-Time PCR platforms.

Quality Control

One-Step Probe RT-qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of DNase and RNase contamination.

This is used for applications such as:

• RT-qPCR
• Gene expression analysis and genetic profiling
• miRNA profiling and quantification
• Mass screening and RNA viral pathogen detection
• Characterization of genetically modified organisms (GMO)