ipsogen WT1 ProfileQuant Kit CE

• Highly sensitive and accurate quantitative assay
• Performance optimized by ELN consortium of experts
• Compliance with EU IVD Directive 98/79/EC
• Easy workflow with ready-to-use solutions

To ensure highest sensitivity, the ipsogen WT1 ProfileQuant Kit has been optimized to detect Wilm's tumor (WT1) and ABL gene transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures  Accurate detection of Wilm's tumor plasmid standards and  Reliable detection of ABL plasmid standards).

The ipsogen WT1 ProfileQuant Kit is a ready-to-use kit for the detection of Wilm's tumor (WT1) gene transcripts using real-time PCR. The kit is based on the amplification and quantification of specific Wilm's tumor (WT1) gene transcripts, relative to ABL control gene expression, in total RNA isolated from patients with acute myeloid leukemia (AML). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen WT1 ProfileQuant Kit includes specific plasmids and primers and probe mixes for WT1 and ABL (see figures  WT1 gene transcript and  ABL control gene transcript). Included are 5 standard dilutions for Wilm's tumor (WT1) and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. These components have been validated together in the context of a collaborative study led by a group of experts from the European Leukemia Net (ELN) consortium.