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QIAamp DNA FFPE Tissue Kit (50)
Cat. No. / ID: 56404
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
Use our next-generation QIAamp DNA FFPE Advanced Kits for improved performance.
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The QIAamp DNA FFPE Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Rapid purification of high-quality, ready-to-use DNA
- Consistent, high yields
- Complete removal of contaminants and inhibitors
Product Details
The QIAamp DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed, paraffin-embedded tissue sections. The kit uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects caused by formalin crosslinking of nucleic acids. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA in small volumes. Purification of DNA using the QIAamp DNA FFPE Tissue Kit can be automated on the QIAcube Connect.
Try the next-generation QIAamp DNA FFPE Advanced Kits for efficient recovery from DNA and optional removal of artefacts.
Performance
The QIAamp DNA FFPE Tissue Kit purifies DNA that can be used in a wide range of downstream applications, such as single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping and pharmacogenomic research. DNA purified with the QIAamp DNA FFPE Tissue Kit can also be used in PCR (see figure " PCR analysis of DNA purified from FFPE tissue samples").
See figures
Principle
The QIAamp DNA FFPE Tissue Kit uses well-established QIAamp MinElute technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl.
Specially optimized lysis conditions allow genomic DNA to be efficiently purified from FFPE tissue sections without the need for overnight incubation. Incubation at an elevated temperature after proteinase K digestion partially removes formalin crosslinking of the released DNA, improving yield as well as DNA performance in downstream assays. Note that DNA isolated from FFPE samples is usually of lower molecular weight than DNA from fresh or frozen samples. The degree of fragmentation depends on the type and age of the sample and the conditions used for fixation.
Procedure
The QIAamp DNA FFPE Tissue procedure consists of 6 steps: remove paraffin, lyse, heat, bind, wash, and elute (see flowchart " Procedure"). After sample lysis, the simple QIAamp DNA FFPE Tissue procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes.
See figures
Applications
The QIAamp DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed paraffin-embedded tissue.
Supporting data and figures
PCR analysis of DNA purified from FFPE tissue samples.
Genomic DNA was purified from different FFPE tissue samples using the QIAamp DNA FFPE Tissue Kit. Products of the prnp gene (78 bp, 464 bp, and 727 bp) were amplified by real-time quantitative PCR.
Specifications
| Features | Specifications |
|---|---|
| Applications | Real-time PCR, STR analysis, LMD-PCR |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Elution volume | 20-100 µl |
| Format | Spin column |
| Technology | Silica technology |
| Main sample type | Formalin fixed paraffin embedded tissue samples |
| Sample amount | Up to 8 sections, each with a thickness of up to 10 µm and a surface area of up to 250 mm2 |
Resources
Brochures & Guides (5)
Kit Handbooks (1)
Safety Data Sheets (1)
Additional Resources (1)
Supplementary Protocols (1)
Certificates of Analysis (1)
Publications
Gender-related survival differences associated with EGFR polymorphisms in metastatic colon cancer.
Cancer Res; 2008; 68 (8):3037-42 2008 Apr 15 PMID:18413774
Discrepancy between CYP2D6 phenotype and genotype derived from post-mortem dextromethorphan blood level.
Forensic Sci Int; 2000; 110 (1):61-70 2000 May 8 PMID:10802201
FAQ
I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?