ipsogen PML-RARA bcr3 Kit

For quantitative detection of PML-RARA bcr3 fusion transcripts using real-time PCR

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ipsogen PML-RARA bcr3 Kit (24)

Cat. No. / ID: 672313

For 24 reactions: ABL Control Gene Standards, PML-RARA bcr3 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix PML-RARA bcr3 Fusion Gene

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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Sensitive and accurate quantitative assay
EAC standardized assay for reproducible results
Easy workflow with ready-to-use solutions

Product Details

The ipsogen PML-RARA bcr3 Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of PML-RARA bcr3 fusion transcripts, relative to ABL control gene expression, in total RNA

Performance

To ensure highest sensitivity, the ipsogen PML-RARA bcr3 kit has been optimized to detect PML-RARA bcr3 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of PML-RARA bcr3 fusion gene plasmid standards and Reliable detection of ABL plasmid standards).

See figures

Reliable detection of ABL plasmid standards.

Principle

The ipsogen PML-RARA bcr3 Kit is a ready-to-use kit for the quantification of PML-RARA bcr3 fusion transcripts using real-time PCR. The kit is based on the amplification and detection of specific PML-RARA bcr3 transcripts, relative to ABL control gene expression, in total RNA (see figures PML-RARA bcr3 gene transcript and ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. Each ipsogen PML-RARA bcr3 Kit provides 5 standard dilutions for PML-RARA bcr3 fusion gene and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. Standardization and validation of the quantification method has been established by Europe Against Cancer (EAC) and the ipsogen PML-RARA bcr3 Kit is based on this technique.

See figures

PML-RARA bcr3 gene transcript.

ABL control gene transcript.

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen PML-RARA bcr3 Kit allows detection and quantification of PML-RARA bcr3 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen PML-RARA bcr3 Kit enable sensitive and reliable detection and quantification of PML-RARA bcr3 transcripts, relative to ABL control gene expression, for research use only.