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ipsogen JAK2 MutaQuant Kit

采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测

S_3159_IpsogenJAK2_s_2

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ipsogen JAK2 MutaQuant Kit (24)

Cat. No. / ID:   673513

For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏的定量检测试剂
  • 准确定量JAK2 V617F/G1849T突变 
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen JAK2 MutaQuant Kit可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒的试剂经过优化,能够灵敏、可靠的检测和定量疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T突变。

绩效

为确保高灵敏度,ipsogen JAK2 MutaQuant Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测JAK2 V617F/G1849T突变(参见 Accurate detection of JAK2 V617F plasmid standards Reliable detection of JAK2 wild-type plasmid standards)。灵敏检测的阈值(LOD)为0.061%。至少含有10,000个拷贝的JAK2基因(野生型和V617F突变型)的样本即可获得理想的检测灵敏度。
查看图表

原理

ipsogen JAK2 MutaQuant Kits为即用型试剂盒,适用于疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T体细胞突变定量检测(由但核苷酸多态性SNP引起),参见 JAK2 mutation site identification。该试剂盒灵敏度高,可重复性强。ipsogen JAK2 MutaQuant Kit为突变的JAK2 V617F提供4个标准品稀释液,为野生型JAK2等位基因提供4个标准品稀释液。使用标准品能够实现准确的定量检测。结果以JAK2 V617F在总JAK2中表达所占的百分比来表示。
查看图表

程序

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaQuant Kit可对JAK2 V617F/G1849T突变进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen JAK2 MutaQuant Kit可对JAK2 V617F/G1849T突变进行定量检测,仅适用于科研。

辅助数据和图表

资源

试剂盒操作手册 (1)
For JAK2 V617F/G1849T quantification
For use with Rotor-Gene Q, ABI PRISM 7900HT SDS, Applied Biosystems 7500 Real-Time PCR System, and LightCycler instruments
For Research Use Only. Not for use in diagnostic procedures.
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)
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