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QIAamp DSP Viral RNA Mini Kit

用于从不含细胞的体液中纯化病毒RNA

S_1084_5_GEN_V2

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QIAamp DSP Viral RNA Mini Kit (50)

Cat. No. / ID:   61904

For 50 RNA preps: QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), and RNase-free Buffers
QIAamp DSP Viral RNA Mini Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 快速分离高品质、即用型RNA
  • 无需有机抽提和乙醇沉淀
  • 产量高且稳定
  • 去除污染物和抑制剂

产品详情

QIAamp DSP Viral RNA Mini Kit提供基于硅胶的病毒RNA纯化和对低病毒滴度的样本进行浓缩。此试剂盒专为处理病毒RNA的实验室设计。

绩效

QIAamp DSP Viral RNA Mini Kit包含QIAamp Mini离心柱,可用于小离心机、在QIAcube全自动核酸纯化仪上自动化或在真空底座中处理(如QIAvac 24 Plus和VacConnectors及VacValves)。样本起始量是140 µl。QIAamp DSP Viral RNA mini Kit纯化的病毒RNA可用于下游应用。

原理

无需酚氯仿抽提。病毒RNA特异性结合在QIAmp硅胶膜上,而污染物可流过。二价阳离子和蛋白质等PCR抑制剂可在两次洗涤步骤中被有效去除,留下的病毒RNA可用纯水或试剂盒中提供的洗脱缓冲液洗脱。

程序

病毒RNA选择性和QIAamp硅胶膜结合,纯化的RNA可用水或试剂盒中提供的洗脱缓冲液洗脱(参见"Procedure")。

应用

QIAamp DSP Viral RNA Mini Kit使用快速离心柱或真空操作流程,简化了从不含细胞的体液、血浆(抗凝血剂处理,而不是肝素处理)或血清中纯化病毒RNA的步骤。

辅助数据和图表

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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