TissueLyser II

用于中到高通量样本破碎，适用于分子分析

Features

破碎过程便利、安全
适配器专为高通量破碎而优化
提供多种配件
确保难裂解组织获得高重复性结果
QIAGEN样本处理体系的重要组成部分

Product Details

通过不锈钢珠、碳化钨珠或玻璃珠在塑料样品管中的高速震动，TissueLyser II可同步破碎多个生物样本。借助适当的适配器，可同时处理48或192个样本。也可使用研磨罐处理大体积样本。同时提供各种研磨珠、研磨珠分配器、收集管及管盖。TissueLyser II包括适配器在内的所有配件与TissueLyser（已停产）兼容。

Performance

TissueLyser II尤其适合高通量的样本破碎，适用于人类、动物和植物组织、细菌和酵母。可纯化获得高品质的DNA、RNA、miRNA和蛋白质，甚至对于难裂解的组织也具有高度的可重复性（参见"Efficient disruption of animal tissues"、"Reproducible RNA purification from plant tissues"、""、""、""和""）。

See figures

Intact protein suitable for all types of analysis.

High-quality DNA from animal tissues.

High-quality DNA from plant tissues.

Reliable detection of miRNAs.

Principle

基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎，以确保获得高产的DNA、RNA和蛋白质。TissueLyser II体系通过研磨珠的高速震荡来实现对样本的有效破碎。TissueLyser II可快速、彻底的破碎样本，彻底释放生物分子，并可同时对样本进行匀，协助后续使用的QIAGEN试剂盒进行纯化（参见"QIAGEN purification kits compatible with QIAGEN disruption systems"）。

QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案，TissueLyser II是其中关键的组成部分。TissueLyser II可作为QIAsymphony SP等QIAGEN自动化仪器高通量制备和分析样本的补充（参见"QIAGEN high-throughput automation"）。这一即用型仪器可从1–96个样本中自动纯化DNA、RNA和蛋白质。TissueLyser II也适用于QIAGEN手动样本制备试剂盒（参见"QIAGEN purification kits compatible with QIAGEN disruption systems"）。

进行RNA研究时，新鲜收集的组织可在RNAlater RNA Stabilization Reagent中稳定，可有效避免操作过程中RNA的降解。对于需要纯化DNA、 RNA和蛋白质的应用，可使用Allprotect Tissue Reagent，将新鲜收集的组织中的待分析物迅速稳定。

QIAGEN高通量自动化仪器
仪器	目的	通量
QIAsymphony SP核酸提取纯化分析仪	纯化DNA、RNA和蛋白质	每批1–96个样本
BioRobot Universal System	纯化DNA和RNA	96孔板
QIAxcel	对DNA片段和RNA进行电泳分析	每批多达96个样本
QIAgility	反应体系构建	每批多达384个样本
Rotor-Gene Q实时荧光定量PCR分析仪	real-time PCR和HRM分析	每批多达100个样本
PyroMark Q96 MD or ID	甲基化和突变分析	每批多达96个样本

与QIAGEN破碎仪器兼容的QIAGEN纯化试剂盒
纯化的被分析物	样本类型	QIAGEN试剂盒
RNA	容易裂解的组织	RNeasy Kits
		RNeasy Plus Kits
		RNeasy Protect Kits
RNA	富含纤维的组织	RNeasy Fibrous Tissue Kits
RNA	各种类型组织	RNeasy Lipid Tissue Kits
RNA	各种类型组织	RNeasy Universal Tissue Kits
RNA	植物组织	RNeasy Plant Mini Kit
RNA	酵母	RNeasy Kits
RNA	细菌	RNeasy Protect Bacteria Kits
microRNA	各种类型组织	miRNeasy Kits
DNA	人类组织	QIAamp DNA Kits
DNA	动物组织	DNeasy Blood & Tissue Kits
蛋白质	组织	Qproteome Mammalian Protein Prep Kit
磷蛋白	组织	PhosphoProtein Purification Kit
糖蛋白	组织	Qproteome Glycoprotein Kits
DNA和RNA	组织	AllPrep DNA/RNA Kits
DNA、RNA和蛋白质	组织	AllPrep DNA/RNA/Protein Mini Kit

Procedure

TissueLyser Adapter Sets使用微型收集管，可处理多达2 x 96个样本，或使用2 ml微型离心管，处理多达2 x 24个样本。如果在样本破碎过程中不使用裂解缓冲液，则需要将适配器置于–80°C环境中预冷。在破碎时样品管需保持密封，以避免交叉污染，这对于real-time RT-PCR或芯片分析等高敏感性的下游应用尤其重要。根据样本类型不同，破碎使用不锈钢珠、碳化钨珠或玻璃珠进行。TissueLyser Bead Dispensers用来方便的将单个珠子加入到离心管中，或同时将96个研磨珠加入到收集管中。TissueLyser II也可配合Grinding Jar Sets使用，破碎大体积样本。

Applications

TissueLyser II每批可处理多达192个样本，因此是遗传学、转录组学和蛋白质组学研究中获得生物信息的理想解决方案。TissueLyser II是新一代高通量测序的首选破碎仪器。TissueLyser II可与多种QIAGEN样本纯化试剂盒配套使用，样本纯化可以手动进行，也可以在QIAcube全自动核酸纯化仪、QIAsymphony SP核酸提取纯化分析仪、EZ1 Advanced、BioRobot或BioSprint工作站上自动进行。

Supporting data and figures

Reproducible RNA purification from plant tissues.

Frozen plant leaves were disrupted using the TissueLyser II (2 x 1 minute). RNA was purified using the RNeasy Plant Mini Kit and analyzed on a 1.2 % formaldehyde agarose gel. The ribosomal RNA bands were sharp and of equal intensity, indicating reproducible purification of intact RNA. T: tomato (100 mg); A: arabidopsis (25 mg); C: cotton (100 mg); M: maize (100 mg); R: rape (100 mg).

Specifications

Features	Specifications
Disruption principle	High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Typical run time	15 sec - 2 x 3 minutes at 15–30 Hz
Technical data	100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Kits compatible with instrument	All kits for purifying RNA/DNA/Protein
Protocol/main application on this intrument	Sample preparation/sample disruption
Throughput	2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
Features	Convenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
Technology	Bead Mill

Resources

FAQ

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.

FAQ ID -374

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143

All TissueLyser II repairs must be done by QIAGEN’s Regional Repair Center. Please contact QIAGEN Technical Service.

FAQ ID -9169

Frequency can be set from 3–30 Hz and is kept constant by a speed controller during operation. It may vary in a range of 0.2–0.4 Hz. If you see larger variations, please make sure not to overload the sample tubes with too much starting material.

If the issue persists, please contact QIAGEN Technical Service to have a repair by QIAGEN’s Regional Repair Center.

FAQ ID -9170

Check assembly of the TissueLyser II adapter. It is very important to assemble the TissueLyser adapter correctly. If done incorrectly, the caps of tubes will not remain tight during the disruption and can result in the leak.

FAQ ID -9171

When a new TissueLyser II is shipped, it has a transport security device located on the underside of the TissueLyser II. This is comprised of 2 screws as shown below. Remove the 2 screws using a screwdriver. Do not discard the screws, as they will need to be reinstalled if the TissueLyser II needs to be transported in the future.

FAQ ID -9172

We do not recommend using the adapters of the TissueLyser II with liquid nitrogen. Instead, the adapters can be precooled at –80°C, either in the freezer or on dry ice.

FAQ ID -9173

We recommend using the stainless steel Grinding Jar Set (cat. no. 69985) for disruption of tough samples, such as bone and teeth. Best results were seen with:
1. Take no more than 1 g of bone
2. Add material to the Grinding Jar Set containing a grinding ball
3. Pour in liquid nitrogen
4. As soon as the liquid nitrogen evaporates, assemble the Grinding Jar Set into the TissueLyser
5. Operate for 1 min at 30 Hz

FAQ ID -9174

We have not specifically tested this application. We suggest using a 7 mm stainless steel bead and disrupting the samples twice at 30 Hz for 2 min each.

Please be advised that these are suggestions only and have not been thoroughly tested by QIAGEN. It is the user’s responsibility to test and further optimize if necessary.

FAQ ID -9175

We do not have instructions or recommendations for you to perform frequency verification on our TissueLyser II. However, our Regional Repair Center can offer this as a service. Please contact QIAGEN Technical Service to request service.

FAQ ID -9176

We have not specifically tested this application. However, several customers have successfully used QIAGEN’s TissueLyser II for disruption of animal tissues to extract proteins. We suggest:

Pre-cool one 5 mm stainless steel bead and the TissueLyser II adapter at 4°C.
Chill the appropriate lysis buffer by incubating at 4°C prior to use
Freeze tissue in dry ice and immediately add 1 cold bead plus 200–300 µL of chilled lysis buffer to the TissueLyser II adapter
Disrupt twice, each time for 30 s at 20 Hz

Please be advised that these are suggestions only and have not been thoroughly tested by QIAGEN. It is the user’s responsibility to test and further optimize if necessary.

FAQ ID -9177

100 mg tissue per sample tube is the maximum amount we recommend when using the 2 x 24 Adapter Set. Larger amounts of tissue would require considered optimization, depending on the tissue type and condition.

When using 100 mg tissue, for best homogenization results, we recommend cutting the sample into 2 pieces of approximately 50 mg each prior to disruption and performing disruption twice, each time at 25 Hz for 5 min.

FAQ ID -9178

Stainless steel beads can be reused for DNA extractions with DNeasy Plant Kits. Used beads can be recovered from cell-debris and cleaned using the procedure below:

Close the cap of the 2 ml microtube and briefly vortex to dislodge the bead and pellet from the bottom of the tube. If using grinding jars for the disruption of large sample volumes, skip to step 2.
Empty the contents of the tubes/jars into a sieve and rinse the beads thoroughly with water.
Incubate beads in 0.4 M HCl for 1 min at room temperature (15–25°C) to degrade any DNA and avoid cross-contamination in future preparations.
Rinse beads thoroughly with distilled water to remove the HCl.
Dry beads before use.

You can also find these instructions in Appendix B of the DNeasy Plant Handbook.

FAQ ID -9179

Stainless steel beads sold by QIAGEN are not guaranteed to be magnetic. However, you may find some lots of the beads to be magnetic, due to small variations in the chemical compositions (which do not affect the disruptive functions of the beads).

FAQ ID -9180

We do not guarantee the stainless steel beads to be RNase free. However, when used according to our specifications for disruption of biological samples, no special treatment is needed to make the beads RNase free. This is because our chaotropic lysis buffers will usually inactivate these and RNases contained in the samples themselves.

FAQ ID -9181

QIAGEN does not carry this part. You can however purchase the O-ring from Retsch. Their part no. is 22.085.0006. Please see http://www.retsch.com/

FAQ ID -9182

Our stainless steel beads and tungsten carbide beads are ready to use.

FAQ ID -9183

The material of the grinding jar as well as the grinding ball is Teflon. The ball has a stainless steel core and a coating of Teflon.

FAQ ID -9184

Unfortunately, this is not possible. The 3 mm beads are not compatible with the 5 mm or 7 mm TissueLyser Single Bead Dispenser, as this could result in dispensation of multiple beads, and/or the dispenser may get blocked.

FAQ ID -9185

Height: 266 mm (up to 524 mm with the hood raised)

Width: 371 mm

Depth: 461 mm

Weight: Approx. 26 kg (without TissueLyser Adapter Set or Grinding Jar Set installed)

FAQ ID -9186

We do not sell 3 mm stainless steel beads for use with our TissueLyser instruments.

FAQ ID -9187

We do not sell ceramic beads or glass beads for use with our TissueLyser II or TissueLyser LT instruments. However, we still sell stainless steal beads and tungsten carbide beads. For more information, please go to this link.

FAQ ID -9189

Yes, the 2 ml Sample Tubes RB (cat. no. 990381) can be used with both TissueLyser II and TissueLyser LT.

FAQ ID -9188