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PyroMark Supplementary Software

用于PyroMark系统体系构建和分析的扩展

Features

  • 友好的用户界面,扩展分析范围
  • 下拉式菜单,确保选择正确的参数
  • 能够运行用户设计的实验,采用最优化的算法优化引物

Product Details

PyroMark辅助软件扩大了焦磷酸测序技术分析的灵敏度和分析范围。这些软件界面直观友好,允许用户对基因分型、等位基因定量、甲基化分析和从头测序的实验设计进行优化,并为测得的序列与用户导入本地数据库中的序列进行比对搭建了框架。

Principle

PyroMark Assay Design SW 2.0

灵活的PyroMark Assay Design Software 2.0优化了焦磷酸测序技术。特有的的算法提供完备的质量控制,确保实验在任意PyroMark仪器上顺利进行,该软件是建议在多年的焦磷酸测序和PCR优化经验之上的。只需点击几下,该软件即可为基因分型、等位基因定量、序列分析和甲基化分析设计PCR和测序引物。对于甲基化分析,该软件会对亚硫酸氢盐转化后序列进行预测,突出CpG位点和可作为对照的非CpG的胞嘧啶。

PyroMark CpG SW 1.0

分析多个样本时,每个样本都可能会有数个甲基化位点,那就需要有一个能够帮助清楚呈现整个序列模式以及特定变异位点详细信息的软件。用于PyroMark Q96 ID和PyroMark Q96 MD 进行CpG分析的PyroMark CpG SW 1.0就有这样的功能。标准的运算包括甲基化频率、质量评估、计算每个孔和附孔的平均甲基化水平、统计多个连续位点的甲基化模式以及预期的甲基化模式的偏差。结果以简单易读的图形和数字呈现。

PyroMark IdentiFire SW 1.0

PyroMark IdentiFire SW 1.0使本地序列数据库的编辑更容易,焦磷酸测序结果与其进行快速匹配。原始数据、匹配记录和每个匹配的一致性百分比都会详细的呈现在鉴定报告上。该软件用于与PyroMark Q96 ID的直接对接,但也可处理从PyroMark Q24输入的文件。

Resources

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