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ipsogen Leukemia
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ipsogen
PML-RARA bcr1 Kit
ipsogen
PML-RARA bcr1 Kit
采用real-time PCR对PML-RARA bcr1融合基因转录本进行定量检测
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ipsogen
PML-RARA bcr1 Kit (24)
Cat. No. / ID:
672113
For 24 reactions: ABL Control Gene Standards, PML-RARA bcr1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix PML-RARA bcr1 Fusion Gene
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏、准确的定量检测试剂
符合EAC要求的标准化试剂,结果具有可重复性
即用型解决方案,便利的工作流程
产品详情
ipsogen
PML-RARA bcr1 Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对PML-RARA bcr1融合基因转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。
绩效
为确保高灵敏度,
ipsogen
PML-RARA bcr1 Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测PML-RARA bcr1和ABL转录本(参见
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards
和
Reliable detection of ABL plasmid standards
)。
查看图表
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards.
Reliable detection of ABL plasmid standards.
PML-RARA bcr1 gene transcript.
ABL control gene transcript.
原理
ipsogen
PML-RARA bcr1 Kit为即用型试剂盒,采用real-time PCR技术,检测PML-RARA bcr1融合基因转录本。该试剂盒可扩增并检测总RNA中相对于ABL对照基因表达的特定PML-RARA bcr1融合基因转录本(参见
PML-RARA bcr1 gene transcript
和
ABL control gene transcript
)。该试剂盒灵敏度高,特异性强,可重复性好。
ipsogen
PML-RARA bcr1 Kit为PML-RARA bcr1融合基因提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。已与欧洲抗癌协会(EAC)联合对检测方法的标准化进行验证,
ipsogen
PML-RARA Kit正是基于这种技术。
查看图表
PML-RARA bcr1 gene transcript.
ABL control gene transcript.
Accurate detection of PML-RARA bcr1 fusion gene plasmid standards.
Reliable detection of ABL plasmid standards.
程序
第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。
ipsogen
PML-RARA bcr1 Kit可对PML-RARA bcr1和ABL转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
PML-RARA bcr1 Ki能够对PML-RARA bcr1转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。
辅助数据和图表
PML-RARA bcr1 gene transcript.
PML-RARA bcr1 gene transcript.
资源
试剂盒操作手册 (1)
(EN) - ipsogen FusionQuant Handbook
EN
PDF
(937KB)
安全数据表 (1)
Safety Data Sheets
EN
Download Safety Data Sheets for QIAGEN product components.
Safety Data Sheets (1)
Safety Data Sheets
EN
Certificates of Analysis (1)
Certificates of Analysis
EN
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