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ipsogen RT Kit

用于总RNA的逆转录实验,仅适用于科研

S_1084_5_GEN_V2

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ipsogen RT Kit (33)

Cat. No. / ID:   679913

For 33 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 用于逆转录的高品质试剂
  • 用于逆转录的可靠试剂
  • 进行准确、可重复性的逆转录

产品详情

ipsogen RT Kit仅适用于科研,不可用于诊断。该试剂盒中的试剂经过优化,能够在Rotor-Gene Q实时荧光定量PCR分析仪或其他PCR仪上准确、可靠的进行逆转录实验。该试剂盒适用于将总RNA逆转录为cDNA,用于下游的real-time PCR应用。

原理

ipsogen RT Kit是即用型试剂盒,可通过逆转录PCR将总RNA逆转录为cDNA。使用该试剂盒进行的逆转录实验具有准确、可重复性高的特点。逆转录酶是具有3个不同酶活性的多功能酶,包括依赖于RNA的DNA聚合酶活性、依赖于杂交的核糖核酸外切酶活性和依赖于DNA的DNA聚合酶活性。在体内,这三种酶活性共同作用,能够将单链RNA逆转录为双链DNA。依赖于RNA的DNA聚合酶活性(逆转录)可实现RNA模板到cDNA的逆转录。因此能够合成用于克隆、PCR或测序的cDNA。

程序

第一步是抽提总RNA,并检测其量与品质。第二步是使用ipsogen RT Kit将总RNA逆转录为cDNA。ipsogen RT Kit可实现总RNA的准确、可靠的逆转录。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen RT Kit可实现总RNA的准确、可靠的逆转录。仅适用于科研。

资源

试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
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