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ipsogen Leukemia
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ipsogen
JAK2...
ipsogen
JAK2 Muta
Quant
Kit
采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测
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ipsogen
JAK2 MutaQuant Kit (24)
Cat. No. / ID:
673513
For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的(再)订购
特点
灵敏的定量检测试剂
准确定量JAK2 V617F/G1849T突变
即用型解决方案,便利的工作流程
产品详情
ipsogen
JAK2 Muta
Quant
Kit可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒的试剂经过优化,能够灵敏、可靠的检测和定量疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T突变。
绩效
为确保高灵敏度,
ipsogen
JAK2 Muta
Quant
Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测JAK2 V617F/G1849T突变(参见
Accurate detection of JAK2 V617F plasmid standards
和
Reliable detection of JAK2 wild-type plasmid standards
)。灵敏检测的阈值(LOD)为0.061%。至少含有10,000个拷贝的JAK2基因(野生型和V617F突变型)的样本即可获得理想的检测灵敏度。
查看图表
Accurate detection of JAK2 V617F plasmid standards.
Reliable detection of JAK2 wild-type plasmid standards.
JAK2 mutation site identification.
原理
ipsogen
JAK2 Muta
Quant
Kits为即用型试剂盒,适用于疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T体细胞突变定量检测(由但核苷酸多态性SNP引起),参见
JAK2 mutation site identification
。该试剂盒灵敏度高,可重复性强。
ipsogen
JAK2 Muta
Quant
Kit为突变的JAK2 V617F提供4个标准品稀释液,为野生型JAK2等位基因提供4个标准品稀释液。使用标准品能够实现准确的定量检测。结果以JAK2 V617F在总JAK2中表达所占的百分比来表示。
查看图表
JAK2 mutation site identification.
Accurate detection of JAK2 V617F plasmid standards.
Reliable detection of JAK2 wild-type plasmid standards.
程序
第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。
ipsogen
JAK2 Muta
Quant
Kit可对JAK2 V617F/G1849T突变进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。
应用
ipsogen
JAK2 Muta
Quant
Kit可对JAK2 V617F/G1849T突变进行定量检测,仅适用于科研。
辅助数据和图表
JAK2 mutation site identification.
JAK2 mutation site identification.
资源
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