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ipsogen JAK2 MutaQuant Kit

采用real-time PCR对JAK2 V617F/G1849T突变进行定量检测

S_3159_IpsogenJAK2_s_2

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ipsogen JAK2 MutaQuant Kit (24)

Cat. No. / ID:   673513

For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
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仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏的定量检测试剂
  • 准确定量JAK2 V617F/G1849T突变 
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen JAK2 MutaQuant Kit可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒的试剂经过优化,能够灵敏、可靠的检测和定量疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T突变。

绩效

为确保高灵敏度,ipsogen JAK2 MutaQuant Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测JAK2 V617F/G1849T突变(参见 Accurate detection of JAK2 V617F plasmid standards Reliable detection of JAK2 wild-type plasmid standards)。灵敏检测的阈值(LOD)为0.061%。至少含有10,000个拷贝的JAK2基因(野生型和V617F突变型)的样本即可获得理想的检测灵敏度。
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原理

ipsogen JAK2 MutaQuant Kits为即用型试剂盒,适用于疑似骨髓增殖性肿瘤(MPN)人士的外周血基因组中的JAK2 V617F/G1849T体细胞突变定量检测(由但核苷酸多态性SNP引起),参见 JAK2 mutation site identification。该试剂盒灵敏度高,可重复性强。ipsogen JAK2 MutaQuant Kit为突变的JAK2 V617F提供4个标准品稀释液,为野生型JAK2等位基因提供4个标准品稀释液。使用标准品能够实现准确的定量检测。结果以JAK2 V617F在总JAK2中表达所占的百分比来表示。
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程序

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaQuant Kit可对JAK2 V617F/G1849T突变进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen JAK2 MutaQuant Kit可对JAK2 V617F/G1849T突变进行定量检测,仅适用于科研。

辅助数据和图表

资源

安全数据表 (1)
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